Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Heavy metals influence on spread and persistance of antibiotic resistance genes in waters |
| Abstract | While bacterial strains that are resistant to antibiotics and/or to heavy metals are commonly found in aquatic environments, the relationship between genic elements of resistance to heavy metals (HMR) and those to antibiotics (AR) within natural bacterial communities is still little understood. Furthermore, the distribution of these genes in the water column and in sediments is still poorly elucidated. The presence of antibiotic resistant bacteria and AR genes in aquatic environments in absence of a selective pressure is already documented, while the same assumption is not confirmed in respect to the resistance to heavy metals. Moreover, it remains unclear if native bacterial communities could adapt to heavy metals pollution by selection of HMR bacteria and if this adaptation could promote the AR spread by the co-selection between HMR and AR genes. With this research we aim to understand the vertical and the spatial distribution of HMR genes, their potential spread within the bacterial community, and their link to AR genes by improved quantitative PCR protocols. We will evaluate the impact of a previous heavy metal pollution on the bacterial community of restored Lake Orta (Italy) by sequencing analysis of 16SrDNA and correlation between composition and presence of resistance genes. |
| Source | Lake Orta: a new lease on life, Verbania Pallanza, 23 settembre 2014 |
| Keywords | Lake OrtaLimingAntibiotic resistance genes |
| Year | 2014 |
| Type | Presentazione |
| Authors | Gianluca Corno |
| Text | 330082 2014 Lake Orta Liming Antibiotic resistance genes Heavy metals influence on spread and persistance of antibiotic resistance genes in waters Gianluca Corno CNR ISE While bacterial strains that are resistant to antibiotics and/or to heavy metals are commonly found in aquatic environments, the relationship between genic elements of resistance to heavy metals HMR and those to antibiotics AR within natural bacterial communities is still little understood. Furthermore, the distribution of these genes in the water column and in sediments is still poorly elucidated. The presence of antibiotic resistant bacteria and AR genes in aquatic environments in absence of a selective pressure is already documented, while the same assumption is not confirmed in respect to the resistance to heavy metals. Moreover, it remains unclear if native bacterial communities could adapt to heavy metals pollution by selection of HMR bacteria and if this adaptation could promote the AR spread by the co selection between HMR and AR genes. With this research we aim to understand the vertical and the spatial distribution of HMR genes, their potential spread within the bacterial community, and their link to AR genes by improved quantitative PCR protocols. We will evaluate the impact of a previous heavy metal pollution on the bacterial community of restored Lake Orta Italy by sequencing analysis of 16SrDNA and correlation between composition and presence of resistance genes. Published version Lake Orta a new lease on life Verbania Pallanza 23 settembre 2014 Internazionale Contributo PPT 2014_PPT_Orta_Corno.pdf Presentazione gianluca.corno CORNO GIANLUCA TA.P04.016.004 Ecologia teorica e applicata degli ecosistemi acquatici |