Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Role of Biofilm in Protection of the Replicative Form of Legionella pneumophila |
| Abstract | The dual nature of Legionella pneumophila enables its survival in free and intracellular environments and underpins its infection and spread mechanisms. Experiments using bacterial cultures and improved RTqPCR protocols were devised to gain fresh insights into the role of biofilm in protecting the replicative form of L. pneumophila. mip gene expression was used as a marker of virulence in sessile (biofilm-bound) and planktonic (free-floating) cells of L. pneumophila serotype 1 ATCC 33152. The ratio of mip gene expression to transcriptionally active Legionella cells increased both in sessile and free-floating cells demonstrating an up-regulation of mip gene under nutrient depletion. However, a different trend was observed between the two forms, in planktonic cells the mip gene expression/transcriptionally active Legionella cells increased until the end of the experiment, while in the biofilm such increase was observed at the end of the experiment. These findings suggest a possible association between the switch to the transmissive phase of Legionella and a mip up-regulation and a role for biofilm in preserving Legionella cells in replicative form. Moreover, it has been shown that improved RTqPCR protocols are valuable tools to explore bacterial virulence. |
| Source | Current microbiology (Print) 69 (6), pp. 769–774 |
| Keywords | Legionella pneumophilaBiofilm |
| Journal | Current microbiology (Print) |
| Editor | Springer-Verl. New York., New York, Stati Uniti d'America |
| Year | 2014 |
| Type | Articolo in rivista |
| DOI | 10.1007/s00284-014-0648-y |
| Authors | Elisa Andreozzi; Andrea Di Cesare; Luigia Sabatini; Elisa Chessa; Davide Sisti; Marco Rocchi; Barbara Citterio |
| Text | 328680 2014 10.1007/s00284 014 0648 y ISI Web of Science WOS 000343913800001 Scopus 2 s2.0 84919902727 Legionella pneumophila Biofilm Role of Biofilm in Protection of the Replicative Form of Legionella pneumophila Elisa Andreozzi; Andrea Di Cesare; Luigia Sabatini; Elisa Chessa; Davide Sisti; Marco Rocchi; Barbara Citterio University of Urbino; Marche Polytechnic University; Consiglio Nazionale delle Ricerche CNR ; University of Urbino The dual nature of Legionella pneumophila enables its survival in free and intracellular environments and underpins its infection and spread mechanisms. Experiments using bacterial cultures and improved RTqPCR protocols were devised to gain fresh insights into the role of biofilm in protecting the replicative form of L. pneumophila. mip gene expression was used as a marker of virulence in sessile biofilm bound and planktonic free floating cells of L. pneumophila serotype 1 ATCC 33152. The ratio of mip gene expression to transcriptionally active Legionella cells increased both in sessile and free floating cells demonstrating an up regulation of mip gene under nutrient depletion. However, a different trend was observed between the two forms, in planktonic cells the mip gene expression/transcriptionally active Legionella cells increased until the end of the experiment, while in the biofilm such increase was observed at the end of the experiment. These findings suggest a possible association between the switch to the transmissive phase of Legionella and a mip up regulation and a role for biofilm in preserving Legionella cells in replicative form. Moreover, it has been shown that improved RTqPCR protocols are valuable tools to explore bacterial virulence. 69 Published version Articolo 2014_Curr_Microbiol_69_769_774.pdf Articolo in rivista Springer Verl. New York. 0343 8651 Current microbiology Print Current microbiology Print Curr. microbiol. Print andrea.dicesare DI CESARE ANDREA TA.P04.016.004 Ecologia teorica e applicata degli ecosistemi acquatici |