| Title | Ostreopsis cf. ovata induces cytoskeletal disorganization, apoptosis and gene expression disregulation on HeLa cells |
| Abstract | The dinoflagellates of the genus Ostreopsis Schmidt are toxic species involved in the occurrence of massive blooms. These dinoflagellates are known to produce palytoxin and ovatoxins, which are considered amongst the most poisonous phycotoxins in the world. In this work, aqueous and methanolic extracts of one O. cf. ovata Ionian strain were tested on the human-derived HeLa cell line, and cytotoxicity, cytoskeletal rearrangment and apoptosis induction were recorded by morphological and molecular analysis. The MTT test revealed high toxicity for both extracts, which activity was inhibited by ouabain, which suggests the main involvement of palytoxin-like molecules in the observed toxic effects of this strain. Overall, the effects induced by O. cf. ovata on HeLa cells were dependent on the type of adopted extracts, being both toxic, but with the methanolic extract more toxic than the aqueous one and able to induce cell death by apoptosis. This process was evidenced by both phosphatidylserine exposition and up regulation of caspase-3 gene expression. The different effect of the two extracts on cell death is indicative of their different composition and/or activity. Our findings represent the starting step for the characterization of novel bioactive molecules. |
| Source | Journal of applied phycology (Dordr., Online) 27 (6), pp. 2321–2332 |
| Keywords | Ostreopsis ovatacytotoxicityapoptosisHeLa cellstoxins |
| Journal | Journal of applied phycology (Dordr., Online) |
| Editor | Kluwer, Dordrecht, Paesi Bassi |
| Year | 2015 |
| Type | Articolo in rivista |
| DOI | 10.1007/s10811-014-0515-z. |
| Authors | Pagliara P., Scarano A., Barca A., Verri T., Zuppone S., Caroppo C. |
| Text | 323531 2015 10.1007/s10811 014 0515 z. ISI Web of Science WOS WOS 000363553600016 Scopus 2 s2.0 84945287779 Ostreopsis ovata cytotoxicity apoptosis HeLa cells toxins Ostreopsis cf. ovata induces cytoskeletal disorganization, apoptosis and gene expression disregulation on HeLa cells Pagliara P., Scarano A., Barca A., Verri T., Zuppone S., Caroppo C. Department of Biological and Environmental Sciences and Technologies, University of Salento, Via Provinciale Lecce Monteroni, 73100 Lecce, Italy; Institute of Experimental Neurology and Division of Neuroscience, Neuropathology Unit, IRCCS San Raffaele Scientific Institute, Italy; National Research Council, Water Research Institute, Via Roma 3, 74100 Taranto, Italy The dinoflagellates of the genus Ostreopsis Schmidt are toxic species involved in the occurrence of massive blooms. These dinoflagellates are known to produce palytoxin and ovatoxins, which are considered amongst the most poisonous phycotoxins in the world. In this work, aqueous and methanolic extracts of one O. cf. ovata Ionian strain were tested on the human derived HeLa cell line, and cytotoxicity, cytoskeletal rearrangment and apoptosis induction were recorded by morphological and molecular analysis. The MTT test revealed high toxicity for both extracts, which activity was inhibited by ouabain, which suggests the main involvement of palytoxin like molecules in the observed toxic effects of this strain. Overall, the effects induced by O. cf. ovata on HeLa cells were dependent on the type of adopted extracts, being both toxic, but with the methanolic extract more toxic than the aqueous one and able to induce cell death by apoptosis. This process was evidenced by both phosphatidylserine exposition and up regulation of caspase 3 gene expression. The different effect of the two extracts on cell death is indicative of their different composition and/or activity. Our findings represent the starting step for the characterization of novel bioactive molecules. 27 Published version https //biblioproxy.cnr.it 2280/article/10.1007/s10811 014 0515 z 20/12/2014 Ostreopsis cf. ovata induces cytoskeletal disorganization, apoptosis and gene expression disregulation on HeLa cells J_A_P_2015.pdf Articolo in rivista Kluwer 1573 5176 Journal of applied phycology Dordr., Online Journal of applied phycology Dordr., Online J. appl. phycol. Dordr., Online Journal of applied phycology. Dordr., Online carmela.caroppo CAROPPO CARMELA TA.P04.002.001 Fascia Costiera e Ambienti di Transizione analisi strutturale e funzionale delle componenti biotiche ed abiotiche degli ecosistemi per lo sviluppo sostenibile delle attivita TA.P04.029.002 Utilizzazione di biomasse e sostanze attive da organismi marini biotecnologie per loo sviluppo di nuovi bio materiali e composti bioattivi |
