Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Role of Myxicola infundibulum (Polychaeta, Annelida) mucus: From bacterial control to nutritional home site |
| Abstract | The polychaete Myxicola infundibulum (Renier) produces a large amount of dense mucus which forms a gelatinous envelope where the worm lives. In the present study we evaluated some of the physical-chemical properties of this matrix such as viscosity, osmolarity, electrical conductivity, and protein, carbohydrate and total lipid content The presence of an antibacterial lysozyme-like activity as well as the density of culturable bacteria at 22 degrees C and vibrios and associated macrofauna were also investigated. The water content of M. infundibulum mucus was 95 +/- 0.5%. By dry weight, 38 +/- 1.2% was protein, 2 +/- 021% was carbohydrate and only 3% was lipid. Most of the remainder of the dry weight (about 57%) was inorganic. The mucus of M. infundibulum exerted a lysozyme-like activity evidenced on Petri dishes inoculated with Micrococcus luteus cell walls with a diameter of lysis of 8.4 +/- 0.3 mm, corresponding to 1.15 mg ml(-1) of hen egg white lysozyme. Notwithstanding this high antibacterial activity, the mucous tubes of M. infundibulum contained a high density of culturable heterotrophic bacteria at 22 degrees C, of which presumptive culturable vibrios accounted for 4.6 +/- 0.2 x 10(3) CPU ml(-1). Moreover, tubes were found to be colonized by several specimens of the polychaete Lumbrineris cfr. latreilli. Therefore the mucus of M. infundibutum appears to provide defence against some bacteria and food supply for the growth of others, that in turn could be utilized by macrofaunal organisms. On account of these features this matrix represents an accessible and renewable resource that could repay further exploration from several points of view. (C) 2014 Elsevier B.V. All rights reserved. |
| Source | Journal of experimental marine biology and ecology 461, pp. 344–349 |
| Keywords | Antibacterial activityCulturable bacteriaLumbrineris cfr. latreilliMacrofaunaMucusMyxicola infundibulum |
| Journal | Journal of experimental marine biology and ecology |
| Editor | Elsevier, Amsterdam, Paesi Bassi |
| Year | 2014 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.jembe.2014.09.005 |
| Authors | Stabili, Loredana; Schirosi, Roberto; Licciano, Margherita; Giangrande, Adriana |
| Text | 318125 2014 10.1016/j.jembe.2014.09.005 ISI Web of Science WOS 000346894900041 Antibacterial activity Culturable bacteria Lumbrineris cfr. latreilli Macrofauna Mucus Myxicola infundibulum Role of Myxicola infundibulum Polychaeta, Annelida mucus From bacterial control to nutritional home site Stabili, Loredana; Schirosi, Roberto; Licciano, Margherita; Giangrande, Adriana Consiglio Nazionale delle Ricerche CNR ; University of Salento The polychaete Myxicola infundibulum Renier produces a large amount of dense mucus which forms a gelatinous envelope where the worm lives. In the present study we evaluated some of the physical chemical properties of this matrix such as viscosity, osmolarity, electrical conductivity, and protein, carbohydrate and total lipid content The presence of an antibacterial lysozyme like activity as well as the density of culturable bacteria at 22 degrees C and vibrios and associated macrofauna were also investigated. The water content of M. infundibulum mucus was 95 / 0.5%. By dry weight, 38 / 1.2% was protein, 2 / 021% was carbohydrate and only 3% was lipid. Most of the remainder of the dry weight about 57% was inorganic. The mucus of M. infundibulum exerted a lysozyme like activity evidenced on Petri dishes inoculated with Micrococcus luteus cell walls with a diameter of lysis of 8.4 / 0.3 mm, corresponding to 1.15 mg ml 1 of hen egg white lysozyme. Notwithstanding this high antibacterial activity, the mucous tubes of M. infundibulum contained a high density of culturable heterotrophic bacteria at 22 degrees C, of which presumptive culturable vibrios accounted for 4.6 / 0.2 x 10 3 CPU ml 1 . Moreover, tubes were found to be colonized by several specimens of the polychaete Lumbrineris cfr. latreilli. Therefore the mucus of M. infundibutum appears to provide defence against some bacteria and food supply for the growth of others, that in turn could be utilized by macrofaunal organisms. On account of these features this matrix represents an accessible and renewable resource that could repay further exploration from several points of view. C 2014 Elsevier B.V. All rights reserved. 461 Published version Articolo in rivista Elsevier 0022 0981 Journal of experimental marine biology and ecology Journal of experimental marine biology and ecology J. exp. mar. biol. ecol. loredana.stabili STABILI LOREDANA TA.P04.029.002 Utilizzazione di biomasse e sostanze attive da organismi marini biotecnologie per loo sviluppo di nuovi bio materiali e composti bioattivi |