| Title | Characterization of Vibrios Diversity in the Mucus of the Polychaete Myxicola infundibulum (Annellida, Polichaeta) |
| Abstract | Vibrios are among the most abundant culturable microbes in aquatic environments. They can be either free-living in the water column or associated with several marine organisms as mutualists, saprophytes, or parasites. In the present study we analysed vibrios abundance and diversity in the mucus of the polychaete Myxicola infundibulum, complementing culture-based with molecular methods. Vibrios reached 4.6 x 10(3) CFU mL(-1) thus representing a conspicuous component of the heterotrophic culturable bacteria. In addition, luminous vibrios accounted for about 60 % of the total culturable vibrios in the mucus. The isolates were assigned to: Vibrio gigantis, Vibrio fischeri, Vibrio jasicida, Vibrio crassostreae, Vibrio kanaloae, and Vibrio xuii. Two Vibrio isolates (MI-13 and MI-15) may belong to a new species. We also tested the ability of the Vibrio isolates to grow on M. infundibulum mucus as the sole carbon source. All strains showed appreciable growth in the presence of mucus, leading us to conclude that this matrix, which is abundant and covers the animal entirely, may represent a microcosm and a food source for some bacteria, playing a crucial role in the structuring of a mucus-associated beneficial microbial community. Moreover, the trophic relationship between vibrios and M. infundibulum mucus could be enhanced by the protection that mucus offers to vibrios. The results of this study represent a contribution to the growing evidence for complex and dynamic invertebrate-microbe associations present in nature and highlight the importance of exploring relationships that Vibrio species establish with marine invertebrates. |
| Source | Microbial ecology 67 (1), pp. 186–194 |
| Keywords | vibriosmucusmyxicola infundibulum |
| Journal | Microbial ecology |
| Editor | Springer-Verlag,, New York,, Stati Uniti d'America |
| Year | 2014 |
| Type | Articolo in rivista |
| DOI | 10.1007/s00248-013-0312-2 |
| Authors | Stabili, Loredana; Giangrande, Adriana; Pizzolante, Graziano; Caruso, Giorgia; Alifano, Pietro |
| Text | 318097 2014 10.1007/s00248 013 0312 2 ISI Web of Science WOS 000330982500018 vibrios mucus myxicola infundibulum Characterization of Vibrios Diversity in the Mucus of the Polychaete Myxicola infundibulum Annellida, Polichaeta Stabili, Loredana; Giangrande, Adriana; Pizzolante, Graziano; Caruso, Giorgia; Alifano, Pietro Consiglio Nazionale delle Ricerche CNR ; University of Salento; University of Salento Vibrios are among the most abundant culturable microbes in aquatic environments. They can be either free living in the water column or associated with several marine organisms as mutualists, saprophytes, or parasites. In the present study we analysed vibrios abundance and diversity in the mucus of the polychaete Myxicola infundibulum, complementing culture based with molecular methods. Vibrios reached 4.6 x 10 3 CFU mL 1 thus representing a conspicuous component of the heterotrophic culturable bacteria. In addition, luminous vibrios accounted for about 60 % of the total culturable vibrios in the mucus. The isolates were assigned to Vibrio gigantis, Vibrio fischeri, Vibrio jasicida, Vibrio crassostreae, Vibrio kanaloae, and Vibrio xuii. Two Vibrio isolates MI 13 and MI 15 may belong to a new species. We also tested the ability of the Vibrio isolates to grow on M. infundibulum mucus as the sole carbon source. All strains showed appreciable growth in the presence of mucus, leading us to conclude that this matrix, which is abundant and covers the animal entirely, may represent a microcosm and a food source for some bacteria, playing a crucial role in the structuring of a mucus associated beneficial microbial community. Moreover, the trophic relationship between vibrios and M. infundibulum mucus could be enhanced by the protection that mucus offers to vibrios. The results of this study represent a contribution to the growing evidence for complex and dynamic invertebrate microbe associations present in nature and highlight the importance of exploring relationships that Vibrio species establish with marine invertebrates. 67 Published version Articolo in rivista Springer Verlag, 0095 3628 Microbial ecology Microbial ecology Microb Ecol Microbial ecology. loredana.stabili STABILI LOREDANA TA.P04.029.002 Utilizzazione di biomasse e sostanze attive da organismi marini biotecnologie per loo sviluppo di nuovi bio materiali e composti bioattivi |
