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TitleApplication of relative real-time PCR to detect differential expression of virulence genes in Vibrio anguillarum under standard and stressed growth conditions
AbstractIn this study, we aimed to understand whether abiotic factors affect the expression of virulence genes in Vibrio anguillarum. We observed the in vitro responses of two Mediterranean strains of V. anguillarum to temperature, NaCl and iron concentration changes. We monitored growth performance and gene transcription levels by comparing the results obtained under stressed conditions (temperatures of 5 °C, 15 °C and 37 °C; NaCl concentrations of 3% and 5%; and iron depletion and excess) with those obtained under standard growth conditions (25 °C, 1.5% NaCl and 0.6 ?m of iron). The results showed that the strains respond differently. The strain 975/I was most strongly affected by conditions of 15 °C and iron depletion; these conditions induced increased transcription levels of empA, angR and fatA. Growth of the strain 17/I was inhibited at 15 °C and in iron depletion conditions; this strain also showed dramatic changes in the transcription levels of toxR and tonB2 under increased NaCl concentrations. These results demonstrate that environmental stress affects the expression of virulence genes in V. anguillarum that have implications for the competitiveness, stress tolerance and the ability of V. anguillarum to cause infection. © 2013 John Wiley & Sons Ltd.
SourceJournal of fish diseases (Print) 37 (7), pp. 629–640
KeywordsGene expressionStress responseVibrio anguillarum
JournalJournal of fish diseases (Print)
EditorBlackwell Scientific Publications., Boston;, Regno Unito
Year2014
TypeArticolo in rivista
DOI10.1111/jfd.12158
AuthorsCrisafi, Francesca; Denaro, Renata; Genovese, Maria; Yakimov, Michail M.; Genovese, Lucrezia
Text313974 2014 10.1111/jfd.12158 Scopus 2 s2.0 84902080784 Gene expression Stress response Vibrio anguillarum Application of relative real time PCR to detect differential expression of virulence genes in Vibrio anguillarum under standard and stressed growth conditions Crisafi, Francesca; Denaro, Renata; Genovese, Maria; Yakimov, Michail M.; Genovese, Lucrezia Istituto per l Ambiente Marino Costiero del Consiglio Nazionale delle Ricerche IAMC CNR In this study, we aimed to understand whether abiotic factors affect the expression of virulence genes in Vibrio anguillarum. We observed the in vitro responses of two Mediterranean strains of V. anguillarum to temperature, NaCl and iron concentration changes. We monitored growth performance and gene transcription levels by comparing the results obtained under stressed conditions temperatures of 5 °C, 15 °C and 37 °C; NaCl concentrations of 3% and 5%; and iron depletion and excess with those obtained under standard growth conditions 25 °C, 1.5% NaCl and 0.6 m of iron . The results showed that the strains respond differently. The strain 975/I was most strongly affected by conditions of 15 °C and iron depletion; these conditions induced increased transcription levels of empA, angR and fatA. Growth of the strain 17/I was inhibited at 15 °C and in iron depletion conditions; this strain also showed dramatic changes in the transcription levels of toxR and tonB2 under increased NaCl concentrations. These results demonstrate that environmental stress affects the expression of virulence genes in V. anguillarum that have implications for the competitiveness, stress tolerance and the ability of V. anguillarum to cause infection. © 2013 John Wiley Sons Ltd. 37 http //www.scopus.com/record/display.url eid=2 s2.0 84902080784 origin=inward Application of relative real time PCR to detect virulence genes differential expression in Vibrio anguillarum under standard and stressed conditions Crisafi_et_al_2014.html Articolo in rivista Blackwell Scientific Publications. 0140 7775 Journal of fish diseases Print Journal of fish diseases Print J. fish dis. Print francesca.crisafi CRISAFI FRANCESCA lucrezia.genovese GENOVESE LUCREZIA mikhail.iakimov IAKIMOV MIKHAIL renata.denaro DENARO RENATA maria.genovese GENOVESE MARIA TA.P04.026.002 Impatto Antropogenico su ecosistemi marini microbiologia ed biotecnologia ambientale