| Title | Biotechnological potential of the seaweed Cladophora rupestris (Chlorophyta, Cladophorales) lipidic extract |
| Abstract | Recently, with the advent of modern technologies, various marine organisms are being studied for their bioactive compounds. Antimicrobial substances secreted by algae are attracting considerable attention due to the necessity of finding new substances effective on both classical microorganisms and new pathogens In the present study the antimicrobial activity of the lipidic extract of the green seaweed Cladophora rupestris collected in a Mediterranean area in two sampling periods (January and April) was assayed. The chemical characterization of the lipidic fractions was performed by gas-cromatatography and multinuclear and multidimensional NMR spectroscopy. In the lipidic extract of C. rupestris collected in January an antibacterial activity against Enterococcus sp., Streptococcus agalactiae and Vibrio cholerae non-O1 was recorded; by contrast, bacterial inhibition was measured on several Vibrio species only in April. The fatty acid profile of C. rupestris lipidic extract, analyzed by gas chromatography, resulted mainly composed of palmitic, myristic, oleic, ? linolenic, palmitoleic and linoleic acids. Moreover, since ?-linolenic acid was the predominant ?3 fatty acid in April, we suggest its involvement in the antibacterial activity observed in this month, taking also into account that pure ?-linolenic acid resulted effective towards vibrios strains. Cladophora rupestris fatty acid profile revealed also an interesting composition in polyunsaturated fatty acids in both the considered periods with the ?6/?3 ratio lower than 1, leading to conclude that this macroalga may be employed as a natural source of ?3. Finally, the 1H NMR spectrum in CDCl3 of algal lipid fractions showed the characteristic signals of saturated (SAFAs) and unsaturated fatty acids (UFAs) as well as other metabolites and a marked difference in free fatty acids (FFAs) content for the two examined algal lipid fractions. It is noteworthy that C. rupestris lipidic extracts show, by NMR spectroscopy, the signal pattern of poly hydroxy butirrate, a natural biocompatible and biodegradable polymer. In conclusion, on account of its antimicrobial activity, nutritional value and bioplastic content, C. rupestris lipidic extract can be considered a promising source for future biotechnological applications. |
| Source | New biotechnology (Online) 31 (5), pp. 436–444 |
| Keywords | Cladophora rupestris; lipidic extract; antimicrobial activity; ?6/?3 ratio; poly hydroxy butirrate |
| Journal | New biotechnology (Online) |
| Editor | Elsevier, Amsterdam, Paesi Bassi |
| Year | 2014 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.nbt.2014.05.002 |
| Authors | Stabili L.; Acquaviva M.I.; Biandolino F.; Cavallo R.A.; De Pascali S.A.; Fanizzi F.P.; Narracci M.; Cecere E.; Petrocelli A. |
| Text | 311610 2014 10.1016/j.nbt.2014.05.002 Scopus 2 s2.0 84903729218 Cladophora rupestris; lipidic extract; antimicrobial activity; 6/ 3 ratio; poly hydroxy butirrate Biotechnological potential of the seaweed Cladophora rupestris Chlorophyta, Cladophorales lipidic extract Stabili L.; Acquaviva M.I.; Biandolino F.; Cavallo R.A.; De Pascali S.A.; Fanizzi F.P.; Narracci M.; Cecere E.; Petrocelli A. Institute for Marine Coastal Environment IAMC , C.N.R., Via Roma 3, 74123 Taranto, Italy; Department of Biological and Environmental Sciences and Technologies, University of Salento, Centro Ecotekne Pal. B, S.P. 6 Lecce Monteroni, 73100 Lecce, Italy Recently, with the advent of modern technologies, various marine organisms are being studied for their bioactive compounds. Antimicrobial substances secreted by algae are attracting considerable attention due to the necessity of finding new substances effective on both classical microorganisms and new pathogens In the present study the antimicrobial activity of the lipidic extract of the green seaweed Cladophora rupestris collected in a Mediterranean area in two sampling periods January and April was assayed. The chemical characterization of the lipidic fractions was performed by gas cromatatography and multinuclear and multidimensional NMR spectroscopy. In the lipidic extract of C. rupestris collected in January an antibacterial activity against Enterococcus sp., Streptococcus agalactiae and Vibrio cholerae non O1 was recorded; by contrast, bacterial inhibition was measured on several Vibrio species only in April. The fatty acid profile of C. rupestris lipidic extract, analyzed by gas chromatography, resulted mainly composed of palmitic, myristic, oleic, linolenic, palmitoleic and linoleic acids. Moreover, since linolenic acid was the predominant 3 fatty acid in April, we suggest its involvement in the antibacterial activity observed in this month, taking also into account that pure linolenic acid resulted effective towards vibrios strains. Cladophora rupestris fatty acid profile revealed also an interesting composition in polyunsaturated fatty acids in both the considered periods with the 6/ 3 ratio lower than 1, leading to conclude that this macroalga may be employed as a natural source of 3. Finally, the 1H NMR spectrum in CDCl3 of algal lipid fractions showed the characteristic signals of saturated SAFAs and unsaturated fatty acids UFAs as well as other metabolites and a marked difference in free fatty acids FFAs content for the two examined algal lipid fractions. It is noteworthy that C. rupestris lipidic extracts show, by NMR spectroscopy, the signal pattern of poly hydroxy butirrate, a natural biocompatible and biodegradable polymer. In conclusion, on account of its antimicrobial activity, nutritional value and bioplastic content, C. rupestris lipidic extract can be considered a promising source for future biotechnological applications. 31 Published version http //www.scopus.com/inward/record.url eid=2 s2.0 84903729218 partnerID=q2rCbXpz Articolo in rivista Elsevier 1876 4347 New biotechnology Online New biotechnology Online New biotechnology. Online antonella.petrocelli PETROCELLI ANTONELLA loredana.stabili STABILI LOREDANA mariaimmacolata.acquaviva ACQUAVIVA MARIA IMMACOLATA marcella.narracci NARRACCI MARCELLA francesca.biandolino BIANDOLINO FRANCESCA ester.cecere CECERE ESTER rosaanna.cavallo CAVALLO ROSA ANNA TA.P04.002.001 Fascia Costiera e Ambienti di Transizione analisi strutturale e funzionale delle componenti biotiche ed abiotiche degli ecosistemi per lo sviluppo sostenibile delle attivita |
