Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Two-dimensional versus three-dimensional morphometry of monogenoidean sclerites |
| Abstract | A new method of three-dimensional (3-D) analysis of sclerotised structures of monogenoids was performed by processing z-series images using 3D-Doctor. Z-series were obtained from Gomori's trichrome-stained specimens of marine and freshwater monogenoids under laser scanning confocal fluorescence microscopy. Measurements obtained from 3-D images were then compared with those from 2-D images taken from both flattened and unflattened specimens. Data comparison demonstrated that 3-D morphometry allowed avoidance of over-estimation due to deformation and the reduction of errors associated with different spatial orientations. Moreover, study of 3-D images permitted observation of morphological details that are not detectable in 2-D representations. (c) 2007 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved. |
| Source | International Journal for Parasitology 37 (3-4), pp. 449–456 |
| Keywords | three-dimensional morphometrylaser scanning confocal fluorescence microscopymonogenoideamonogeneanKuhnia scombriHaliotrema curvipenisDactylogyrus extensus |
| Journal | International Journal for Parasitology |
| Editor | Pergamon Press., Oxford, Regno Unito |
| Year | 2007 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.ijpara.2006.11.017 |
| Authors | Galli, Paolo; Strona, Giovanni; Villa, Anna Maria; Benzoni, Francesca; Stefani, Fabrizio; Doglia, Silvia Maria; Kritsky, Delane C. |
| Text | 307851 2007 10.1016/j.ijpara.2006.11.017 ISI Web of Science WOS 000244771600020 three dimensional morphometry laser scanning confocal fluorescence microscopy monogenoidea monogenean Kuhnia scombri Haliotrema curvipenis Dactylogyrus extensus Two dimensional versus three dimensional morphometry of monogenoidean sclerites Galli, Paolo; Strona, Giovanni; Villa, Anna Maria; Benzoni, Francesca; Stefani, Fabrizio; Doglia, Silvia Maria; Kritsky, Delane C. University of Milano Bicocca; Idaho State University A new method of three dimensional 3 D analysis of sclerotised structures of monogenoids was performed by processing z series images using 3D Doctor. Z series were obtained from Gomori s trichrome stained specimens of marine and freshwater monogenoids under laser scanning confocal fluorescence microscopy. Measurements obtained from 3 D images were then compared with those from 2 D images taken from both flattened and unflattened specimens. Data comparison demonstrated that 3 D morphometry allowed avoidance of over estimation due to deformation and the reduction of errors associated with different spatial orientations. Moreover, study of 3 D images permitted observation of morphological details that are not detectable in 2 D representations. c 2007 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved. 37 Articolo in rivista Pergamon Press. 0020 7519 International Journal for Parasitology International Journal for Parasitology Int. J. Parasitol. fabrizio.stefani STEFANI FABRIZIO |