Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Phosphatase activities of cultured phototrophic biofilms |
| Abstract | The responses of cultured phototrophic biofilms to diverse phosphorus (P) regimes were assessed using a semi-continuous flow incubator. Three biofilms were grown over 18 days under three different P regimes: replete inorganic P, organic P-only and limited inorganic P. Assessing the response of the biofilms took into account the rate of phosphomonoesterase and phosphodiesterase activities, biofilm nutrient contents and biomass accrual across the growth period. Phosphorus limitation was indicated by slower biomass accumulation and higher phosphatase activities of the organic P-only and P-limited biofilms compared to the P-replete biofilms. The cyanobacterium Phormidium sp. dominated the later stages in all the treatments forming a dense layer at the biofilm-medium interface. This layer possibly led to a reduction of light and nutrient diffusion to sub-surface cells and may account for the production of phosphatases under P replete conditions. In addition, the Phormidium-layer possibly produced a top-heavy P (and N) distribution and could explain the large reductions in areal nutrient concentrations. End-product repression and de-repression of phosphatase activity was suggested to be a main controlling factor of phosphatase activity. Consequently, it is proposed that for efficient nutrient removal from wastewaters that biofilms should be regularly removed to continually maintain biofilms at the initial stages (3-7 days). © 2011 Elsevier Ltd. |
| Source | Water research (Oxf.) 46 (2), pp. 378–386 |
| Keywords | Organic phosphorusPhosphodiesterasePhosphomonoesterasePhosphorus contentPhosphorus limitationWastewater treatment |
| Journal | Water research (Oxf.) |
| Editor | Pergamon Press., New York, Regno Unito |
| Year | 2012 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.watres.2011.10.057 |
| Authors | Ellwood N.T.W.; Di Pippo F.; Albertano P. |
| Text | 300712 2012 10.1016/j.watres.2011.10.057 Scopus 2 s2.0 84355161483 Organic phosphorus Phosphodiesterase Phosphomonoesterase Phosphorus content Phosphorus limitation Wastewater treatment Phosphatase activities of cultured phototrophic biofilms Ellwood N.T.W.; Di Pippo F.; Albertano P. Department of Geological Sciences, University of Rome Roma Tre, Largo San Leonardo Murialdo 1, 00146 Rome, Italy; CNR IAMC, National Research Council, Institute for Coastal Marine Environment, Localita Sa Mardini, Torregrande, 09072 Oristano, Italy; LBA Laboratory for Biology of Algae, Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica snc, 00133 Rome, Italy The responses of cultured phototrophic biofilms to diverse phosphorus P regimes were assessed using a semi continuous flow incubator. Three biofilms were grown over 18 days under three different P regimes replete inorganic P, organic P only and limited inorganic P. Assessing the response of the biofilms took into account the rate of phosphomonoesterase and phosphodiesterase activities, biofilm nutrient contents and biomass accrual across the growth period. Phosphorus limitation was indicated by slower biomass accumulation and higher phosphatase activities of the organic P only and P limited biofilms compared to the P replete biofilms. The cyanobacterium Phormidium sp. dominated the later stages in all the treatments forming a dense layer at the biofilm medium interface. This layer possibly led to a reduction of light and nutrient diffusion to sub surface cells and may account for the production of phosphatases under P replete conditions. In addition, the Phormidium layer possibly produced a top heavy P and N distribution and could explain the large reductions in areal nutrient concentrations. End product repression and de repression of phosphatase activity was suggested to be a main controlling factor of phosphatase activity. Consequently, it is proposed that for efficient nutrient removal from wastewaters that biofilms should be regularly removed to continually maintain biofilms at the initial stages 3 7 days . © 2011 Elsevier Ltd. 46 http //www.scopus.com/inward/record.url eid=2 s2.0 84355161483 partnerID=q2rCbXpz Articolo in rivista Pergamon Press. 0043 1354 Water research Oxf. Water research Oxf. Water res. Oxf. Water research. Oxf. francesca.dipippo DI PIPPO FRANCESCA |