Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Thermophilic anaerobic digestion of thermal pretreated sludge: Role of microbial community structure and correlation with process performances |
| Abstract | Thermal hydrolysis pretreatment coupled with Thermophilic Anaerobic Digestion (TAD) for Waste Activated Sludge (WAS) treatment is a promising combination to improve biodegradation kinetics during stabilization. However, to date there is a limited knowledge of the anaerobic biomass composition and its impact on TAD process performances.In this study, the structure and dynamics of the microbial communities selected in two semi-continuous anaerobic digesters, fed with untreated and thermal pretreated sludge, were investigated. The systems were operated for 250 days at different organic loading rate.16S rRNA gene clonal analysis and Fluorescence In Situ Hybridization (FISH) analyses allowed us to identify the majority of bacterial and archaeal populations. Proteolytic Coprothermobacter spp. and hydrogenotrophic Methanothermobacter spp. living in strict syntrophic association were found to dominate in TAD process.The establishment of a syntrophic proteolytic pathway was favoured by the high temperature of the process and enhanced by the thermal pretreatment of the feeding sludge. Proteolytic activity, alone or with thermal pretreatment, occurred during TAD as proven by increasing concentration of soluble ammonia and soluble COD (sCOD) during the process. However, the availability of a readily biodegradable substrate due to pretreatment allowed to significant sCOD removals (more than 55%) corresponding to higher biogas production in the reactor fed with thermal pretreated sludge. Microbial population dynamics analysed by FISH showed that Coprothermobacter and Methanothermobacter immediately established a stable syntrophic association in the reactor fed with pretreated sludge in line with the overall improved TAD performances observed under these conditions. |
| Source | Water research (Oxf.) 68, pp. 498–509 |
| Keywords | CoprothermobacterFluorescence in situ hybridizationMethanothermobacterThermal hydrolysisThermophilic anaerobic digestion |
| Journal | Water research (Oxf.) |
| Editor | Pergamon Press., New York, Regno Unito |
| Year | 2015 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.watres.2014.10.031 |
| Authors | Gagliano, Maria Cristina; Braguglia, Camilla Maria; Gianico, A.; Mininni, Giuseppe; Nakamura, K.; Rossetti, Simona |
| Text | 300084 2015 10.1016/j.watres.2014.10.031 ISI Web of Science WOS 000347756900045 Scopus 2 s2.0 84910088715 Coprothermobacter Fluorescence in situ hybridization Methanothermobacter Thermal hydrolysis Thermophilic anaerobic digestion Thermophilic anaerobic digestion of thermal pretreated sludge Role of microbial community structure and correlation with process performances Gagliano, Maria Cristina; Braguglia, Camilla Maria; Gianico, A.; Mininni, Giuseppe; Nakamura, K.; Rossetti, Simona Water Research Institute CNR IRSA; Gifu University Thermal hydrolysis pretreatment coupled with Thermophilic Anaerobic Digestion TAD for Waste Activated Sludge WAS treatment is a promising combination to improve biodegradation kinetics during stabilization. However, to date there is a limited knowledge of the anaerobic biomass composition and its impact on TAD process performances.In this study, the structure and dynamics of the microbial communities selected in two semi continuous anaerobic digesters, fed with untreated and thermal pretreated sludge, were investigated. The systems were operated for 250 days at different organic loading rate.16S rRNA gene clonal analysis and Fluorescence In Situ Hybridization FISH analyses allowed us to identify the majority of bacterial and archaeal populations. Proteolytic Coprothermobacter spp. and hydrogenotrophic Methanothermobacter spp. living in strict syntrophic association were found to dominate in TAD process.The establishment of a syntrophic proteolytic pathway was favoured by the high temperature of the process and enhanced by the thermal pretreatment of the feeding sludge. Proteolytic activity, alone or with thermal pretreatment, occurred during TAD as proven by increasing concentration of soluble ammonia and soluble COD sCOD during the process. However, the availability of a readily biodegradable substrate due to pretreatment allowed to significant sCOD removals more than 55% corresponding to higher biogas production in the reactor fed with thermal pretreated sludge. Microbial population dynamics analysed by FISH showed that Coprothermobacter and Methanothermobacter immediately established a stable syntrophic association in the reactor fed with pretreated sludge in line with the overall improved TAD performances observed under these conditions. 68 Published version http //www.scopus.com/record/display.url eid=2 s2.0 84910088715 origin=inward Thermophilic anaerobic digestion of thermal pretreated sludge Role of microbial community structure and correlation with process performances Manuscript 2015 Gagliano et al WR.pdf Articolo in rivista Pergamon Press. 0043 1354 Water research Oxf. Water research Oxf. Water res. Oxf. Water research. Oxf. GAGLIANO MARIA CRISTINA simona.rossetti ROSSETTI SIMONA camillamaria.braguglia BRAGUGLIA CAMILLA MARIA giuseppe.mininni MININNI GIUSEPPE andrea.gianico GIANICO ANDREA ROUTES Novel processing routes for effective sewage sludge management |