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DatoValore
TitlePHA production by mixed cultures: A way to valorize wastes from pulp industry
AbstractIn this work, hardwood spent sulfite liquor (HSSL), a complex feedstock originating from the pulp industry, was tested for the first time as a substrate for polyhydroxyalkanoate (PHA) production by a mixed microbial culture (MMC) under aerobic dynamic feeding (ADF) conditions. A sequencing batch reactor (SBR) fed with HSSL was operated for 67. days and the selected MMC reached a maximum PHA content of 67.6%. The MMC demonstrated a differential utilization of the carbon sources present in HSSL. Acetic acid was fully consumed, while xylose and lignosulphonates were partially consumed (30% and 14%, respectively). The selected culture was characterized by Fluorescence in Situ Hybridization (FISH). Bacteria belonging to the three main classes were identified: Alpha- (72.7. ±. 4.0%), Beta- (11.1. ±. 0.37%) and Gammaproteobacteria (10.3. ±. 0.3%). Within Alphaproteobacteria, a small amount of Paracoccus (4.2. ±. 0.51%) and Defluvicoccus related to Tetrad Forming Organisms (9.0. ±. 0.28%) were detected. © 2014 Elsevier Ltd.
SourceBioresource technology 157, pp. 197–205
KeywordsAerobic dynamic feedingFISHHSSLMixed microbial culturesPolyhydroxyalkanoatesactivate sludge
JournalBioresource technology
EditorElsevier Applied Science,, Barking, Regno Unito
Year2014
TypeArticolo in rivista
DOI10.1016/j.biortech.2014.01.099
AuthorsQueiros D.; Rossetti S.; Serafim L.S.
Text300079 2014 10.1016/j.biortech.2014.01.099 ISI Web of Science WOS 2 s2.0 84894040340 Aerobic dynamic feeding FISH HSSL Mixed microbial cultures Polyhydroxyalkanoates activate sludge PHA production by mixed cultures A way to valorize wastes from pulp industry Queiros D.; Rossetti S.; Serafim L.S. CICECO, Departamento de Quimica, Universidade de Aveiro, Campus Universitario de Santiago, P 3810 193 Aveiro, Portugal; Water Research Institute CNR, Via Salaria Km 29, 300, 00015 Monterotondo, Italy In this work, hardwood spent sulfite liquor HSSL , a complex feedstock originating from the pulp industry, was tested for the first time as a substrate for polyhydroxyalkanoate PHA production by a mixed microbial culture MMC under aerobic dynamic feeding ADF conditions. A sequencing batch reactor SBR fed with HSSL was operated for 67. days and the selected MMC reached a maximum PHA content of 67.6%. The MMC demonstrated a differential utilization of the carbon sources present in HSSL. Acetic acid was fully consumed, while xylose and lignosulphonates were partially consumed 30% and 14%, respectively . The selected culture was characterized by Fluorescence in Situ Hybridization FISH . Bacteria belonging to the three main classes were identified Alpha 72.7. ±. 4.0% , Beta 11.1. ±. 0.37% and Gammaproteobacteria 10.3. ±. 0.3% . Within Alphaproteobacteria, a small amount of Paracoccus 4.2. ±. 0.51% and Defluvicoccus related to Tetrad Forming Organisms 9.0. ±. 0.28% were detected. © 2014 Elsevier Ltd. 157 Published version http //www.scopus.com/inward/record.url eid=2 s2.0 84894040340 partnerID=q2rCbXpz PHA production by mixed cultures a way to valorize wastes from pulp industry Pdf della pubblicazione Queir_s_et_al_2014.pdf Articolo in rivista Elsevier Applied Science, 0960 8524 Bioresource technology Bioresource technology Bioresour. technol. Bioresource technology. simona.rossetti ROSSETTI SIMONA