Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | In situ identification of the synthrophic protein fermentative Coprothermobacter spp. involved in the thermophilic anaerobic digestion process |
| Abstract | Thermophilic bacteria have recently attracted great attention because of their potential application in improving different biochemical processes such as anaerobic digestion of various substrates, wastewater treatment or hydrogen production. In this study we report on the design of a specific 16S rRNA-targeted oligonucleotide probe for detecting members of Coprothermobacter genus characterized by a strong protease activity to degrade proteins and peptides. The newly designed CTH485 probe and helper probes hCTH429 and hCTH439 were optimized for use in fluorescence in situ hybridization (FISH) on thermophilic anaerobic sludge samples. In situ probing revealed that thermo-adaptive mechanisms shaping the 16S rRNA gene may affect the identification of thermophilic microorganisms. The novel developed FISH probe extends the possibility to study the widespread thermophilic syntrophic interaction of Coprothermobacter spp. with hydrogenotrophic methanogenic archaea, whose establishment is a great benefit for the whole anaerobic system. © 2014 Federation of European Microbiological Societies. |
| Source | FEMS microbiology letters 358, pp. 55–63 |
| Keywords | CoprothermobacterFISHProteolytic bacteriaSyntrophyThermophilic anaerobic digestion |
| Journal | FEMS microbiology letters |
| Editor | Elsevier/North-Holland, Amsterdam, Paesi Bassi |
| Year | 2014 |
| Type | Articolo in rivista |
| DOI | 10.1111/1574-6968.12528 |
| Authors | Gagliano M.C.; Braguglia C.M.; Rossetti S. |
| Text | 300077 2014 10.1111/1574 6968.12528 Scopus 2 s2.0 84906532090 Coprothermobacter FISH Proteolytic bacteria Syntrophy Thermophilic anaerobic digestion In situ identification of the synthrophic protein fermentative Coprothermobacter spp. involved in the thermophilic anaerobic digestion process Gagliano M.C.; Braguglia C.M.; Rossetti S. Water Research Institute IRSA CNR Monterotondo, RM Italy Thermophilic bacteria have recently attracted great attention because of their potential application in improving different biochemical processes such as anaerobic digestion of various substrates, wastewater treatment or hydrogen production. In this study we report on the design of a specific 16S rRNA targeted oligonucleotide probe for detecting members of Coprothermobacter genus characterized by a strong protease activity to degrade proteins and peptides. The newly designed CTH485 probe and helper probes hCTH429 and hCTH439 were optimized for use in fluorescence in situ hybridization FISH on thermophilic anaerobic sludge samples. In situ probing revealed that thermo adaptive mechanisms shaping the 16S rRNA gene may affect the identification of thermophilic microorganisms. The novel developed FISH probe extends the possibility to study the widespread thermophilic syntrophic interaction of Coprothermobacter spp. with hydrogenotrophic methanogenic archaea, whose establishment is a great benefit for the whole anaerobic system. © 2014 Federation of European Microbiological Societies. 358 Published version http //www.scopus.com/inward/record.url eid=2 s2.0 84906532090 partnerID=q2rCbXpz In situ identification of the synthrophic protein fermentative Coprothermobacter spp. involved in the thermophilic anaerobic digestion process pdf pubblicazione FEMS_2014.pdf Articolo in rivista Elsevier/North Holland 0378 1097 FEMS microbiology letters FEMS microbiology letters FEMS microbiol. lett. FEMS microbiology letters. Federation of European Microbiological Societies microbiology letters GAGLIANO MARIA CRISTINA simona.rossetti ROSSETTI SIMONA camillamaria.braguglia BRAGUGLIA CAMILLA MARIA ROUTES Novel processing routes for effective sewage sludge management |