Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Characterization of tetA-like gene encoding for a major facilitator superfamily efflux pump in Streptococcus thermophilus |
| Abstract | Efflux pumps are membrane proteins involved in the active extrusion of a wide range of structurally dissimilar substrates from cells. A multidrug efflux pump named TetA belonging to the major facilitator superfamily (MFS) of transporters was identified in the Streptococcus thermophilus DSM 20617T genome. The tetA-like gene was found in the genomes of a number of S.thermophilus strains sequenced to date and in Streptococcus macedonicus ACA-DC 198, suggesting a possible horizontal gene transfer event between these two Streptococcus species, which are both adapted to the milk environment. Flow cytometry (single-cell) analysis revealed bistable TetA activity in the S.thermophilus population, and tetA-like gene over-expression resulted in a reduced susceptibility to ethidium bromide, tetracycline, and other toxic compounds even when the efflux pump was over-expressed in a strain naturally lacking tetA-like gene. |
| Source | FEMS microbiology letters 355 (1), pp. 61–70 |
| Keywords | antibiotic resistancelactic acid bacteriaMFS efflux pumpStreptococcus thermophilus |
| Journal | FEMS microbiology letters |
| Editor | Elsevier/North-Holland, Amsterdam, Paesi Bassi |
| Year | 2014 |
| Type | Articolo in rivista |
| DOI | 10.1111/1574-6968.12449 |
| Authors | Arioli, Stefania; Guglielmetti, Simone; Amalfitano, Stefano; Viti, Carlo; Marchi, Emmanuela; Decorosi, Francesca; Giovannetti, Luciana; Mora, Diego |
| Text | 297583 2014 10.1111/1574 6968.12449 ISI Web of Science WOS 000337592600008 antibiotic resistance lactic acid bacteria MFS efflux pump Streptococcus thermophilus Characterization of tetA like gene encoding for a major facilitator superfamily efflux pump in Streptococcus thermophilus Arioli, Stefania; Guglielmetti, Simone; Amalfitano, Stefano; Viti, Carlo; Marchi, Emmanuela; Decorosi, Francesca; Giovannetti, Luciana; Mora, Diego University of Milan; Ist Ric Acque IRSA CNR; University of Florence Efflux pumps are membrane proteins involved in the active extrusion of a wide range of structurally dissimilar substrates from cells. A multidrug efflux pump named TetA belonging to the major facilitator superfamily MFS of transporters was identified in the Streptococcus thermophilus DSM 20617T genome. The tetA like gene was found in the genomes of a number of S.thermophilus strains sequenced to date and in Streptococcus macedonicus ACA DC 198, suggesting a possible horizontal gene transfer event between these two Streptococcus species, which are both adapted to the milk environment. Flow cytometry single cell analysis revealed bistable TetA activity in the S.thermophilus population, and tetA like gene over expression resulted in a reduced susceptibility to ethidium bromide, tetracycline, and other toxic compounds even when the efflux pump was over expressed in a strain naturally lacking tetA like gene. 355 Articolo in rivista Elsevier/North Holland 0378 1097 FEMS microbiology letters FEMS microbiology letters FEMS microbiol. lett. FEMS microbiology letters. Federation of European Microbiological Societies microbiology letters stefano.amalfitano AMALFITANO STEFANO |