Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Snow Surface Microbiome on the High Antarctic Plateau (DOME C) |
| Abstract | The cryosphere is an integral part of the global climate system and one of the major habitable ecosystems of Earth's biosphere. These permanently frozen environments harbor diverse, viable and metabolically active microbial populations that represent almost all the major phylogenetic groups. In this study, we investigated the microbial diversity in the surface snow surrounding the Concordia Research Station on the High Antarctic Plateau through a polyphasic approach, including direct prokaryotic quantification by flow cytometry and catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH), and phylogenetic identification by 16S RNA gene clone library sequencing and 454 16S amplicon pyrosequencing. Although the microbial abundance was low (<10(3) cells/ml of snowmelt), concordant results were obtained with the different techniques. The microbial community was mainly composed of members of the Alpha-proteobacteria class (e.g. Kiloniellaceae and Rhodobacteraceae), which is one of the most well-represented bacterial groups in marine habitats, Bacteroidetes (e.g. Cryomorphaceae and Flavobacteriaceae) and Cyanobacteria. Based on our results, polar microorganisms could not only be considered as deposited airborne particles, but as an active component of the snowpack ecology of the High Antarctic Plateau. |
| Source | PloS one 9 (8) |
| Journal | PloS one |
| Editor | Public Library of Science, San Francisco, CA, Stati Uniti d'America |
| Year | 2014 |
| Type | Articolo in rivista |
| DOI | 10.1371/journal.pone.0104505 |
| Authors | Michaud, Luigi; Lo Giudice, Angelina; Mysara, Mohamed; Monsieurs, Pieter; Raffa, Carmela; Leys, Natalie; Amalfitano, Stefano; Van Houdt, Rob |
| Text | 297539 2014 10.1371/journal.pone.0104505 ISI Web of Science WOS 000339993900071 Snow Surface Microbiome on the High Antarctic Plateau DOME C Michaud, Luigi; Lo Giudice, Angelina; Mysara, Mohamed; Monsieurs, Pieter; Raffa, Carmela; Leys, Natalie; Amalfitano, Stefano; Van Houdt, Rob University of Messina; Belgian Nucl Res Ctr SCK CEN; Vrije University of Brussels; Natl Res Council IRSA CNR The cryosphere is an integral part of the global climate system and one of the major habitable ecosystems of Earth s biosphere. These permanently frozen environments harbor diverse, viable and metabolically active microbial populations that represent almost all the major phylogenetic groups. In this study, we investigated the microbial diversity in the surface snow surrounding the Concordia Research Station on the High Antarctic Plateau through a polyphasic approach, including direct prokaryotic quantification by flow cytometry and catalyzed reporter deposition fluorescence in situ hybridization CARD FISH , and phylogenetic identification by 16S RNA gene clone library sequencing and 454 16S amplicon pyrosequencing. Although the microbial abundance was low <10 3 cells/ml of snowmelt , concordant results were obtained with the different techniques. The microbial community was mainly composed of members of the Alpha proteobacteria class e.g. Kiloniellaceae and Rhodobacteraceae , which is one of the most well represented bacterial groups in marine habitats, Bacteroidetes e.g. Cryomorphaceae and Flavobacteriaceae and Cyanobacteria. Based on our results, polar microorganisms could not only be considered as deposited airborne particles, but as an active component of the snowpack ecology of the High Antarctic Plateau. 9 Articolo in rivista Public Library of Science 1932 6203 PloS one PloS one PLoS ONE PloS one Public Library of Science one PLoS 1 stefano.amalfitano AMALFITANO STEFANO angelina.logiudice LO GIUDICE ANGELINA |