| Title | Capability of the natural microbial community in a river water ecosystem to degrade the drug naproxen |
| Abstract | The present work aims at evaluating the ability of the River Tiber natural microbial community to degrade naproxen in water samples collected downstream from a wastewater treatment plant. For this purpose, different water microcosms were set up (microbiologically active vs sterile ones) and treated with naproxen (100 ug/L) alone or in the co-presence of gemfibrozil in order to evaluate if the co-presence of the latter had an influence on naproxen degradation. The experiment was performed in the autumn and was compared with the same experimental set performed in spring of the same year to highlight if seasonal differences in the river water influenced the naproxen degradation. Pharmaceutical concentrations and microbial analysis (total cell number, viability, and microbial community composition) were performed at different times in the degradation experiments. The overall results show that the natural microbial community in the river water had a key role in the naproxen degradation. In fact, although there was a transient negative effect on the natural microbial community in all the experiments (3 h after adding the pharmaceutical), the latter was able to degrade naproxen within about 40 days. On the contrary, no decrease in the pharmaceutical concentration was observed in the sterile river water. Moreover, the co-presence of the two drugs lengthened the naproxen lag phase. As regards the natural microbial community composition detected by Fluorescence in situ Hybridization, Alpha and Gamma-Proteobacteria increased when the pharmaceutical halved, suggesting their role in the degradation. This study shows that with the concentration studied, naproxen was degraded by the natural microbial populations collected from a river chronically contaminated by this pharmaceutical. |
| Source | Environmental science and pollution research international 21 (23), pp. 13470–13479 |
| Keywords | Degradative bacterial groupsGemfibrozilMicrobial phylogenetic characterizationMicrocosmsNaproxen biodegradationNatural microbial communityRiver Tiber |
| Journal | Environmental science and pollution research international |
| Editor | Springer, Berlin, Germania |
| Year | 2014 |
| Type | Articolo in rivista |
| DOI | 10.1007/s11356-014-3276-y |
| Authors | Grenni P, Patrolecco L, Ademollo N, Di Lenola M, Barra Caracciolo A |
| Text | 297005 2014 10.1007/s11356 014 3276 y Scopus 2 s2.0 84912047225 Degradative bacterial groups Gemfibrozil Microbial phylogenetic characterization Microcosms Naproxen biodegradation Natural microbial community River Tiber Capability of the natural microbial community in a river water ecosystem to degrade the drug naproxen Grenni P, Patrolecco L, Ademollo N, Di Lenola M, Barra Caracciolo A Water Research Institute, National Research Council of Italy, Via Salaria Km 29.300, Monterotondo St., Rome, 00015, Italy The present work aims at evaluating the ability of the River Tiber natural microbial community to degrade naproxen in water samples collected downstream from a wastewater treatment plant. For this purpose, different water microcosms were set up microbiologically active vs sterile ones and treated with naproxen 100 ug/L alone or in the co presence of gemfibrozil in order to evaluate if the co presence of the latter had an influence on naproxen degradation. The experiment was performed in the autumn and was compared with the same experimental set performed in spring of the same year to highlight if seasonal differences in the river water influenced the naproxen degradation. Pharmaceutical concentrations and microbial analysis total cell number, viability, and microbial community composition were performed at different times in the degradation experiments. The overall results show that the natural microbial community in the river water had a key role in the naproxen degradation. In fact, although there was a transient negative effect on the natural microbial community in all the experiments 3 h after adding the pharmaceutical , the latter was able to degrade naproxen within about 40 days. On the contrary, no decrease in the pharmaceutical concentration was observed in the sterile river water. Moreover, the co presence of the two drugs lengthened the naproxen lag phase. As regards the natural microbial community composition detected by Fluorescence in situ Hybridization, Alpha and Gamma Proteobacteria increased when the pharmaceutical halved, suggesting their role in the degradation. This study shows that with the concentration studied, naproxen was degraded by the natural microbial populations collected from a river chronically contaminated by this pharmaceutical. 21 Published version http //www.scopus.com/inward/record.url eid=2 s2.0 84912047225 partnerID=q2rCbXpz Articolo pubblicato Grenni_etal_2014_Capability_of_the_natural_microbial_community_in_a_river_water_ecosystem_to_degrade_the_drug_Naproxen_EnvSciPollRes.pdf Articolo in rivista Springer 0944 1344 Environmental science and pollution research international Environmental science and pollution research international Environ. sci. pollut. res. int. Environmental science and pollution research international. Environmental science and pollution research international Print Environmental science and pollution research Print ESPR Print luisa.patrolecco PATROLECCO LUISA anna.barracaracciolo BARRA CARACCIOLO ANNA paola.grenni GRENNI PAOLA nicoletta.ademollo ADEMOLLO NICOLETTA TA.P04.005.011 Vulnerabilita degli ecosistemi delle acque sotterranee e attenuazione naturale degli inquinanti nel suolo e nel sottosuolo |
