Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Adhesion of marine cryptic Escherichia isolates to human intestinal epithelial cells |
| Abstract | Five distinct cryptic lineages (clades I-V) have recently been recognized in the Escherichia genus. The five clades encompass strains that are phenotypically and taxonomically indistinguishable from Escherichia coli sensu stricto; however, scant data are available on their ecology, virulence and pathogenic properties. In this study 20 cryptic E. coli strains isolated from marine sediments were investigated to gain insights into their virulence characteristics and genetic traits. The ability to adhere to intestinal cells was highest among clade V strains, which also harbored the genes involved in gut colonization as well as the genes (pduC and eut operon) typically found in environmentally adapted E. coli strains. The pduC gene was significantly associated with clade V. Multilocus sequence typing of three representative clade V isolates revealed new sequence types (STs) and showed that the strains shared two allelic loci (adk 51 and recA 37). Our findings suggest that cryptic Escherichia lineages are common in coastal marine sediments and that this habitat may be suitable for their growth and persistence outside the host. On the other hand, detection in clade V strains of a gene repertoire and adhesion properties similar to those of intestinal pathogenic strains could indicate their potential virulence. It could be argued that there is a dual nature of cryptic clade V strains, where the ability to survive and persist in a secondary habitat does not involve the loss of the host-associated lifestyle. Clade V could be a group of closely related, environmentally adapted E. coli strains.The ISME Journal advance online publication, 12 September 2014; doi:10.1038/ismej.2014.164. |
| Source | The ISME journal (Print) 9, pp. 508–515 |
| Keywords | marine sedimentsEscherichia |
| Journal | The ISME journal (Print) |
| Editor | Nature Publishing Group,, London, Regno Unito |
| Year | 2015 |
| Type | Articolo in rivista |
| DOI | 10.1038/ismej.2014.164 |
| Authors | Vignaroli C.; Di Sante L.; Magi G.; Luna G.M.; Di Cesare A.; Pasquaroli S.; Facinelli B.; Biavasco F. |
| Text | 291291 2015 10.1038/ismej.2014.164 Scopus 2 s2.0 84911911111 marine sediments Escherichia Adhesion of marine cryptic Escherichia isolates to human intestinal epithelial cells Vignaroli C.; Di Sante L.; Magi G.; Luna G.M.; Di Cesare A.; Pasquaroli S.; Facinelli B.; Biavasco F. Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona, Italy ISMAR CNR, Arsenale Tesa 104, Venezia Five distinct cryptic lineages clades I V have recently been recognized in the Escherichia genus. The five clades encompass strains that are phenotypically and taxonomically indistinguishable from Escherichia coli sensu stricto; however, scant data are available on their ecology, virulence and pathogenic properties. In this study 20 cryptic E. coli strains isolated from marine sediments were investigated to gain insights into their virulence characteristics and genetic traits. The ability to adhere to intestinal cells was highest among clade V strains, which also harbored the genes involved in gut colonization as well as the genes pduC and eut operon typically found in environmentally adapted E. coli strains. The pduC gene was significantly associated with clade V. Multilocus sequence typing of three representative clade V isolates revealed new sequence types STs and showed that the strains shared two allelic loci adk 51 and recA 37 . Our findings suggest that cryptic Escherichia lineages are common in coastal marine sediments and that this habitat may be suitable for their growth and persistence outside the host. On the other hand, detection in clade V strains of a gene repertoire and adhesion properties similar to those of intestinal pathogenic strains could indicate their potential virulence. It could be argued that there is a dual nature of cryptic clade V strains, where the ability to survive and persist in a secondary habitat does not involve the loss of the host associated lifestyle. Clade V could be a group of closely related, environmentally adapted E. coli strains.The ISME Journal advance online publication, 12 September 2014; doi 10.1038/ismej.2014.164. 9 Published version http //www.scopus.com/inward/record.url eid=2 s2.0 84911911111 partnerID=q2rCbXpz Articolo in rivista Nature Publishing Group, 1751 7362 The ISME journal Print The ISME journal Print ISME j. Print The ISME journal Print International Society for Microbial Ecology journal Print gianmarco.luna LUNA GIAN MARCO andrea.dicesare DI CESARE ANDREA PB.P02.001.003 Progetto Ritmare CdS 080 |