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TitleAlcanivorax borkumensis produces an extracellular siderophore in iron-limitation condition maintaining the hydrocarbon-degradation efficiency
AbstractObligate marine hydrocarbonoclastic bacteria possess genetic and physiological features to use hydrocarbons as sole source of carbon and to compete for the uptake of nutrients in usually nutrient-depleted marine habitats. In the present work we have studied the siderophore-based iron uptake systems in Alcanivorax borkumensis SK2 and their functioning during biodegradation of an aliphatic hydrocarbon, tetradecane, under iron limitation conditions. The antiSMASH analysis of SK2 genome revealed the presence of two different putative operons of siderophore synthetases. Search for the predicted core structures indicated that one siderophore is clearly affiliated to the family of complex oligopeptidic siderophores possessing an Orn-Ser-Orn carboxyl motif whereas the second one is likely to belong to the family of SA (salicylic acid)-based siderophores.Analyzing the supernatant of SK2 culture, an extracellular siderophore was identified and its structure was resolved. Thus, along with the recently described membrane-associated amphiphilic tetrapeptidic siderophore amphibactin, strain SK2 additionally produces an extracellular type of iron-chelating molecule with structural similarity to pseudomonins. Comparative Q-PCR analysis of siderophore synthetases demonstrated their significant up-regulation in iron-depleted medium. Different expression patterns were recorded for two operons during the early and late exponential phases of growth, suggesting a different function of these two siderophores under iron-depleted conditions.
SourceMarine genomics (Print) 17, pp. 43–52
KeywordsBacterial iron uptakeMarine oil-pollutionNon-ribosomal peptide synthetaseReal time Q-PCR
JournalMarine genomics (Print)
EditorElsevier, Amsterdam, Paesi Bassi
Year2014
TypeArticolo in rivista
DOI10.1016/j.margen.2014.07.004
AuthorsDenaro R.; Crisafi F.; Russo D.; Genovese M.; Messina E.; Genovese L.; Carbone M.; Ciavatta M.L.; Ferrer M.; Golyshin P.; Yakimov M.M.
Text288996 2014 10.1016/j.margen.2014.07.004 Scopus 2 s2.0 84908179634 Bacterial iron uptake Marine oil pollution Non ribosomal peptide synthetase Real time Q PCR Alcanivorax borkumensis produces an extracellular siderophore in iron limitation condition maintaining the hydrocarbon degradation efficiency Denaro R.; Crisafi F.; Russo D.; Genovese M.; Messina E.; Genovese L.; Carbone M.; Ciavatta M.L.; Ferrer M.; Golyshin P.; Yakimov M.M. Institute for Coastal Marine Environment CNR, Sp.tA S. Raineri 86, Messina, 98122, Italy; Institute of Biomolecular Chemistry CNR, Via Campi Flegrei, 34, Pozzuoli, Napoli, 80078, Italy; CSIC, Institute of Catalysis, Marie Curie 2, Madrid, 28049, Spain; School of Biological Sciences, Bangor University, Gwynedd, LL57 2UW, United Kingdom Obligate marine hydrocarbonoclastic bacteria possess genetic and physiological features to use hydrocarbons as sole source of carbon and to compete for the uptake of nutrients in usually nutrient depleted marine habitats. In the present work we have studied the siderophore based iron uptake systems in Alcanivorax borkumensis SK2 and their functioning during biodegradation of an aliphatic hydrocarbon, tetradecane, under iron limitation conditions. The antiSMASH analysis of SK2 genome revealed the presence of two different putative operons of siderophore synthetases. Search for the predicted core structures indicated that one siderophore is clearly affiliated to the family of complex oligopeptidic siderophores possessing an Orn Ser Orn carboxyl motif whereas the second one is likely to belong to the family of SA salicylic acid based siderophores.Analyzing the supernatant of SK2 culture, an extracellular siderophore was identified and its structure was resolved. Thus, along with the recently described membrane associated amphiphilic tetrapeptidic siderophore amphibactin, strain SK2 additionally produces an extracellular type of iron chelating molecule with structural similarity to pseudomonins. Comparative Q PCR analysis of siderophore synthetases demonstrated their significant up regulation in iron depleted medium. Different expression patterns were recorded for two operons during the early and late exponential phases of growth, suggesting a different function of these two siderophores under iron depleted conditions. 17 http //www.scopus.com/inward/record.url eid=2 s2.0 84908179634 partnerID=q2rCbXpz Alcanivorax borkumensis produces an extracellular siderophore in iron limitation condition maintaining the hydrocarbon degradation efficiency Denaro_et_al_Marine_genomics_2014.pdf Articolo in rivista Elsevier 1874 7787 Marine genomics Print Marine genomics Print Marine genomics. Print MESSINA ENZO francesca.crisafi CRISAFI FRANCESCA RUSSO DANIELA marialetizia.ciavatta CIAVATTA MARIA LETIZIA mikhail.iakimov IAKIMOV MIKHAIL renata.denaro DENARO RENATA maria.genovese GENOVESE MARIA marianna.carbone CARBONE MARIANNA PM.P01.020.001 Esplorazione della diversita chimica in organismi marini in funzione del drug discovery