Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Adaptations to semi-synthetic antibiotics drive drastic changes in aquatic bacterial communities |
| Abstract | The spread of resistances to commonly used synthetic antibiotics (AB) in natural environments is one of the major threats to human health. A large number of publications investigated the relations between different human pathogens and the spread of AB resistances, but a comparably low number of studies targeted the ecological effect of the presence of synthetic AB on non-pathogenic bacteria in waters. We tested the long-term impact of a cocktail of synthetic antibiotics of commercial use (Imipenem, Levofloaxcin, and Tetracycline) on artificial bacterial communities miming a very simplified natural freshwater community. The experimental setup consisted in a 24 days-long continuous culture system where the bacterial communities were exposed to three different AB concentrations: no AB (control), sub-lethal AB concentration (comparable to anthropized European lakes), and in AB concentration 10 folds higher (comparable to heavily contaminated water treatment plants). Bacterial number and morphological distribution were daily monitored (epifluorescence microscopy), while community composition (CARD-FISH) and antibiotic resistance were assessed bi-weekly. Bacterial production, and thus abundance, dropped by 75% in the presence of AB, independently by their concentration, while bacterial community composition resulted highly affected by intermediate AB concentration, with reversal in the proportion of the dominant groups (Aeromonas hydrophila and Brevundimonas sp.). These results, for the first time focussing on the ecological consequences of AB on a experimental bacterial community, highlight the potential risk posed by low AB concentrations in waters. Their effect is not only limited to the spread of potentially pathogenic strains, but it extends to modification of interspecific ecological interactions, weakening the natural resistance of the aquatic communities to the success of allochtonous, and potentially dangerous, strains. |
| Source | SAME13: EMBO Conference on Aquatic Microbial Ecology, Stresa (VB), 8-13 settembre 2013 |
| Keywords | Freshwater bacteriaAntibioticResistanceChemostats |
| Year | 2013 |
| Type | Poster |
| Authors | Gianluca Corno; Marcol Giardina; Sonia Plechuk; Manuela Coci; Floriana Campanile; Stefania Stefani |
| Text | 284824 2013 Freshwater bacteria Antibiotic Resistance Chemostats Adaptations to semi synthetic antibiotics drive drastic changes in aquatic bacterial communities Gianluca Corno; Marcol Giardina; Sonia Plechuk; Manuela Coci; Floriana Campanile; Stefania Stefani Gianluca Corno; Manuela Coci CNR ISE VB Marcol Giardina; Sonia Plechuk; Floriana Campanile; Stefania Stefani University of Catania, Department of Bio Medical Sciences The spread of resistances to commonly used synthetic antibiotics AB in natural environments is one of the major threats to human health. A large number of publications investigated the relations between different human pathogens and the spread of AB resistances, but a comparably low number of studies targeted the ecological effect of the presence of synthetic AB on non pathogenic bacteria in waters. We tested the long term impact of a cocktail of synthetic antibiotics of commercial use Imipenem, Levofloaxcin, and Tetracycline on artificial bacterial communities miming a very simplified natural freshwater community. The experimental setup consisted in a 24 days long continuous culture system where the bacterial communities were exposed to three different AB concentrations no AB control , sub lethal AB concentration comparable to anthropized European lakes , and in AB concentration 10 folds higher comparable to heavily contaminated water treatment plants . Bacterial number and morphological distribution were daily monitored epifluorescence microscopy , while community composition CARD FISH and antibiotic resistance were assessed bi weekly. Bacterial production, and thus abundance, dropped by 75% in the presence of AB, independently by their concentration, while bacterial community composition resulted highly affected by intermediate AB concentration, with reversal in the proportion of the dominant groups Aeromonas hydrophila and Brevundimonas sp. . These results, for the first time focussing on the ecological consequences of AB on a experimental bacterial community, highlight the potential risk posed by low AB concentrations in waters. Their effect is not only limited to the spread of potentially pathogenic strains, but it extends to modification of interspecific ecological interactions, weakening the natural resistance of the aquatic communities to the success of allochtonous, and potentially dangerous, strains. Published version In SAME13 I EMBO Conference on Aquatic Microbial Ecology Stresa VB , 8 13 settembre 2013 . SAME13 EMBO Conference on Aquatic Microbial Ecology Stresa VB 8 13 settembre 2013 Internazionale Contributo Poster 2013_POSTER_Same_Corno.pdf Poster gianluca.corno CORNO GIANLUCA TA.P04.016.004 Ecologia teorica e applicata degli ecosistemi acquatici |