| Title | Integrative taxonomy does not support the occurrence of two species of the Squalius squalus complex (Actinopterygii, Cypriniformes, Cyprinidae) in Italy |
| Abstract | The systematic is still unresolved for the genus Squalius (Cyprinidae, Leuciscinae), a rich group of small to large fishes widely distributed throughout Europe. The distinction of one of the Italian narrowly endemic species, Squalius albus (Bonaparte, 1838), described for the area surrounding lake Trasimeno, from the more common and widespread Squalius squalus (Bonaparte, 1837) is doubtful. The application of integrative taxonomy, with DNA taxonomy and quantitative morphometric, using both living and preserved individuals collected from lake Trasimeno before Squalius sp. restocking, allowed us to explicitly test for the identity of the two species in the complex. COI barcoding data, used for phylogenetic reconstructions, underlined that two clades may exist in the complex; nevertheless, DNA taxonomy (ABGD and GMYC) and morphometrics show no statistical support for their identity as separate species. Moreover, during our survey of the genetic diversity of the Italian Squalius, we provided further support for the species status of Squalius lucumonis, and found evidence of the occurrence in Southern Italy of another chub species, Squalius vardarensis (Karaman, 1928), previously known only from the Southern part of the Balkan Peninsula. © 2014 Elsevier Ltd. |
| Source | Biochemical systematics and ecology 56, pp. 281–288 |
| Keywords | COICytochrome oxidase IDNA barcodingDNA taxonomyMuseum specimensSqualius albus |
| Journal | Biochemical systematics and ecology |
| Editor | Pergamon Press., New York, Regno Unito |
| Year | 2014 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.bse.2014.07.005 |
| Authors | Lucentini, Livia; Chiesa, Stefania; Giannetto, Daniela; Pompei, Laura; Natali, Mauro; Sala, Paolo; Volta, Pietro; Lorenzoni, Massimo; Fontaneto, Diego |
| Text | 283525 2014 10.1016/j.bse.2014.07.005 Scopus 2 s2.0 84904890441 COI Cytochrome oxidase I DNA barcoding DNA taxonomy Museum specimens Squalius albus Integrative taxonomy does not support the occurrence of two species of the Squalius squalus complex Actinopterygii, Cypriniformes, Cyprinidae in Italy Lucentini, Livia; Chiesa, Stefania; Giannetto, Daniela; Pompei, Laura; Natali, Mauro; Sala, Paolo; Volta, Pietro; Lorenzoni, Massimo; Fontaneto, Diego Universita degli Studi di Perugia; Universidade de Aveiro; Province of Perugia; Consiglio Nazionale delle Ricerche The systematic is still unresolved for the genus Squalius Cyprinidae, Leuciscinae , a rich group of small to large fishes widely distributed throughout Europe. The distinction of one of the Italian narrowly endemic species, Squalius albus Bonaparte, 1838 , described for the area surrounding lake Trasimeno, from the more common and widespread Squalius squalus Bonaparte, 1837 is doubtful. The application of integrative taxonomy, with DNA taxonomy and quantitative morphometric, using both living and preserved individuals collected from lake Trasimeno before Squalius sp. restocking, allowed us to explicitly test for the identity of the two species in the complex. COI barcoding data, used for phylogenetic reconstructions, underlined that two clades may exist in the complex; nevertheless, DNA taxonomy ABGD and GMYC and morphometrics show no statistical support for their identity as separate species. Moreover, during our survey of the genetic diversity of the Italian Squalius, we provided further support for the species status of Squalius lucumonis, and found evidence of the occurrence in Southern Italy of another chub species, Squalius vardarensis Karaman, 1928 , previously known only from the Southern part of the Balkan Peninsula. © 2014 Elsevier Ltd. 56 Published version http //www.sciencedirect.com/science/article/pii/S0305197814001975 Lucentini et al 2014 2014_BiochSystEcol_Lucentini_et_al.pdf Articolo in rivista Pergamon Press. 0305 1978 Biochemical systematics and ecology Biochemical systematics and ecology Biochem. syst. ecol. Biochemical systematics and ecology. pietro.volta VOLTA PIETRO diego.fontaneto FONTANETO DIEGO |
