Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Long-term picocyanobacteria development in an athalassohaline warm-monomictic Lake Alchichica: Virus- vs. ciliate-control? |
| Abstract | Autotrophic picoplankton (APP) was monitored regularly in Lake Alchichica (Puebla/Veracruz; Mexico) for nearly 10 years. In the present study, the control of APP development by viruses is analysed. The maximum cyanobacteria abundance was localised within the water column using WET-Labs ECO Triplet fluorometer and oxygen production by 2µm fraction was measured at four depths using clear & dark bottles. Autofluorescence and DAPI staining combined with an image analysis (Image J) were applied to evaluate APP and bacterial biomass, respectively. The Sherrs´ fluorescently labelled bacteria-protocol was employed to evaluate a role of ciliates. Viruses were estimated microscopically as "virus like particles" according to SYBR Gold protocol, taking CCD camera images. APP were peaking (over 106 cells/mL) during transition between mixing- and stratification period, being responsible for important oxygen production only within the epilimnion; Synechococcus strains were molecularly identified in the isolates. Viruses (107 to 108 VLP/mL) peaked in the surface layer during mixing or in the metalimnion, being coupled apparently more tightly with APP than with bacteria numbers. Both elevated ciliates' APP elimination during mixing period, and the maximum APP elimination around the oxycline in the late stratification roughly coincided with VLP maxima. |
| Source | SAME13: EMBO Conference on Aquatic Microbial Ecology, Stresa (VB), 8-13 settembre 2013 |
| Keywords | Lake AlchichicaAutotrophic picoplanktonVirusProtozoa |
| Year | 2013 |
| Type | Poster |
| Authors | Miroslav Macek; Jorge R. Montiel Hernandez; Aramis E. Florez Marquez; Cristiana Callieri |
| Text | 282645 2013 Lake Alchichica Autotrophic picoplankton Virus Protozoa Long term picocyanobacteria development in an athalassohaline warm monomictic Lake Alchichica Virus vs. ciliate control Miroslav Macek; Jorge R. Montiel Hernandez; Aramis E. Florez Marquez; Cristiana Callieri Miroslav Macek; Jorge R. Montiel Hernandez FES Iztacala, Department of Tropical Limnology, UNAM, Mexico Aramis E. Florez Marquez Technological Institute of Huixquilucan, Mexico Cristiana Callieri CNR ISE VB Autotrophic picoplankton APP was monitored regularly in Lake Alchichica Puebla/Veracruz; Mexico for nearly 10 years. In the present study, the control of APP development by viruses is analysed. The maximum cyanobacteria abundance was localised within the water column using WET Labs ECO Triplet fluorometer and oxygen production by 2µm fraction was measured at four depths using clear dark bottles. Autofluorescence and DAPI staining combined with an image analysis Image J were applied to evaluate APP and bacterial biomass, respectively. The Sherrs´ fluorescently labelled bacteria protocol was employed to evaluate a role of ciliates. Viruses were estimated microscopically as virus like particles according to SYBR Gold protocol, taking CCD camera images. APP were peaking over 106 cells/mL during transition between mixing and stratification period, being responsible for important oxygen production only within the epilimnion; Synechococcus strains were molecularly identified in the isolates. Viruses 107 to 108 VLP/mL peaked in the surface layer during mixing or in the metalimnion, being coupled apparently more tightly with APP than with bacteria numbers. Both elevated ciliates APP elimination during mixing period, and the maximum APP elimination around the oxycline in the late stratification roughly coincided with VLP maxima. Published version In SAME13 I EMBO Conference on Aquatic Microbial Ecology Stresa VB , 8 13 settembre 2013 . SAME13 EMBO Conference on Aquatic Microbial Ecology Stresa VB 8 13 settembre 2013 Internazionale Contributo Poster POSTER_SAME_Macek.pdf Poster cristiana.callieri CALLIERI CRISTIANA TA.P04.016.004 Ecologia teorica e applicata degli ecosistemi acquatici |