| Title | Entero viruses in domestic wells of the Salento groundwater (Southern Italy) |
| Abstract | The Salento (Puglia) carbonate aquifer is an important water reservoir which presents an high vulnerability to the wastewater pollution due to numerous vertical fractures of the rock aquifer. An experimental study has been carried out in the Nardò aquifer (Salento region), where the channel Asso outflows rainfall and other waste's drainages in a natural sinkhole (i.e., karst cavity). The study for qualitative and quantitative viral estimations, was supported by a groundwater monitoring in wells carried out by CNR-IRSA during winter 2002. The collected water samples were analyzed for viral contamination tests by using the culture cell method at Bari University and the RT-nested PCR at Pisa University. Seventeen water samples from selected wells located downgradient the sinkhole with respect to groundwater flow, resulted positive at the RT-PCR and were analysed by cell methods in order to perform differential viral analyses. Three cell lines (BGM, Hep-2 and L20B) were seeded with water samples following two protocols: A and B. Following the protocol A, the water samples were only subjected to antibiotics pretreatment, whereas following the protocol B, the samples were also 10% chloroform treated. The two combined virological methods revealed ground water contamination by Poliovirus 2 (10-5.5CCID50/25mL) and Echovirus 11 (10-5 CCID50/25 mL) in two samples from a well 500 m from the sinkhole and by Poliovirus 3 (10-5CCID50/25mL) in sampled water from a well 3000 m from contamination. The results deserve considerations and suggest severe virological controls of the water from domestic wells water of the Salento. |
| Source | CONGRESSO DELLA SOCIETÀ ITALIANA VIROLOGIA (SIV), ORVIETO, 2004 |
| Keywords | Natural water qualityWellsWaterborne disease outbreaksPathogenic microorganisms detectionNatural water disinfection |
| Year | 2004 |
| Type | Contributo in atti di convegno |
| Authors | VENTOLA, C., CALVARIO, A., MASCIOPINTO, C., SCARASCIULLI, M.,L., SATALINO, M., JATTA, E. |
| Text | 243376 2004 Natural water quality Wells Waterborne disease outbreaks Pathogenic microorganisms detection Natural water disinfection Entero viruses in domestic wells of the Salento groundwater Southern Italy VENTOLA, C., CALVARIO, A., MASCIOPINTO, C., SCARASCIULLI, M.,L., SATALINO, M., JATTA, E. DI.M.I.M.P., Universita di Bari. Consiglio Nazionale delle Ricerche, Istituto di Ricerca Sulle Acque, Bari. Istituto Superiore della Sanita Roma The Salento Puglia carbonate aquifer is an important water reservoir which presents an high vulnerability to the wastewater pollution due to numerous vertical fractures of the rock aquifer. An experimental study has been carried out in the Nardo aquifer Salento region , where the channel Asso outflows rainfall and other waste s drainages in a natural sinkhole i.e., karst cavity . The study for qualitative and quantitative viral estimations, was supported by a groundwater monitoring in wells carried out by CNR IRSA during winter 2002. The collected water samples were analyzed for viral contamination tests by using the culture cell method at Bari University and the RT nested PCR at Pisa University. Seventeen water samples from selected wells located downgradient the sinkhole with respect to groundwater flow, resulted positive at the RT PCR and were analysed by cell methods in order to perform differential viral analyses. Three cell lines BGM, Hep 2 and L20B were seeded with water samples following two protocols A and B. Following the protocol A, the water samples were only subjected to antibiotics pretreatment, whereas following the protocol B, the samples were also 10% chloroform treated. The two combined virological methods revealed ground water contamination by Poliovirus 2 10 5.5CCID50/25mL and Echovirus 11 10 5 CCID50/25 mL in two samples from a well 500 m from the sinkhole and by Poliovirus 3 10 5CCID50/25mL in sampled water from a well 3000 m from contamination. The results deserve considerations and suggest severe virological controls of the water from domestic wells water of the Salento. CONGRESSO DELLA SOCIETÀ ITALIANA VIROLOGIA SIV ORVIETO 2004 Internazionale Su invito Contributo in atti di convegno costantino.masciopinto MASCIOPINTO COSTANTINO |
