| Abstract | The molecular identification of seven biofilm-forming cyanobacteria and the characterization of their exopolysaccharides were made and considered in terms of potential biotechnological applications. The studied strains were isolated from phototrophic biofilms taken from various Italian sites including a wastewater treatment plant, an eroded soil, and a brackish lagoon. The polysaccharides were characterized by use of ion exchange chromatography, circular dichroism, and cytochemical stains. All strains produced exopolysaccharides with differing ratios of hydrophobic and hydrophilic moieties depending on the species, the polysaccharide fraction (i.e., whether capsular or released), and the ambient conditions. It was shown that the anionic nature of the exopolysaccharides was due to the presence of carboxylic and sulfated groups and is likely the main characteristic with industrial applicability. Potential biotechnological applications are discussed. |
| Text | 240303 2013 10.1007/s10811 013 0028 1 Scopus 84887462155 Cyanobacteria Released polysaccharides Capsular polysaccharides EPS Biotechnological applications Characterization of exopolysaccharides produced by seven biofilm forming cyanobacterial strains for biotechnological applications Francesca Di Pippo, Neil T. W. Ellwood, Alessandra Gismondi, Laura Bruno, Federico Rossi, Paolo Magni, Roberto De Philippis CNR IAMC, National Research Council, Institute for Coastal Marine Environment, Localita Sa Mardini, Torregrande, Oristano, Italy; CNR IRSA, National Research Council, Water Research Institute, Area della Ricerca di Roma 1, Monterotondo stazione, Rome, Italy; Department of Geological Sciences, University of Rome Roma Tre , Rome, Italy; LBA Laboratory for Biology of Algae, Department of Biology, University of Rome Tor Vergata , Rome, Italy; Department of Agrifood Production and Environmental Sciences, University of Florence, Florence, Italy; CNR ISMAR, National Research Council, Institute of Marine Science, Venice, Italy The molecular identification of seven biofilm forming cyanobacteria and the characterization of their exopolysaccharides were made and considered in terms of potential biotechnological applications. The studied strains were isolated from phototrophic biofilms taken from various Italian sites including a wastewater treatment plant, an eroded soil, and a brackish lagoon. The polysaccharides were characterized by use of ion exchange chromatography, circular dichroism, and cytochemical stains. All strains produced exopolysaccharides with differing ratios of hydrophobic and hydrophilic moieties depending on the species, the polysaccharide fraction i.e., whether capsular or released , and the ambient conditions. It was shown that the anionic nature of the exopolysaccharides was due to the presence of carboxylic and sulfated groups and is likely the main characteristic with industrial applicability. Potential biotechnological applications are discussed. 25 http //link.springer.com/article/10.1007/s10811 013 0028 1 JAP 2013 JAP_2013.pdf Articolo in rivista Kluwer Academic Publishers 0921 8971 Journal of applied phycology Journal of applied phycology J. appl. phycol. Journal of applied phycology. paolo.magni MAGNI PAOLO francesca.dipippo DI PIPPO FRANCESCA TA.P05.017.002 Ecologia delle aree marino costiere e di transizione processi e funzioni |
