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DatoValore
TitleAssessment of the bacterial community structure in a Brazilian clay soil treated with atrazine
AbstractIn the present paper, the bacterial communities in two soils, one from an agricultural sugarcane cropped field and the other from an unperturbed soil with similar geopedological characteristics, were characterized using the Fluorescence In Situ Hybridization (FISH) method. FISH consists of in situ identification of bacteria using fluorescent labeled 16S rRNA targeted oligonucleotide probes visualizable under epifluorescence microscope. In the cultivated soil, in line with agricultural practice, the pre-emergence herbicide atrazine had been regularly applied each year at a concentration of 5 L/ha. The Shannon Diversity and Evenness Indices were also calculated using the phylogenetic data obtained from the FISH analysis. Although, at the sampling time (6 months after soil atrazine treatment), no residual herbicide concentration was found, the overall bacterial community results show a lower diversity and evenness in the agricultural soil than in the unperturbed one, demonstrating how microbiological indicators are sensitive to anthropogenic disturbance. In the natural soil, the dominant groups were a-Proteobacteria, ?-Proteobacteria, and g-Proteobacteria (representing more than 50 % of the bacteria), but in the agricultural soil, their abundance decreased significantlyand represented just 31 % of the bacteria domain.
SourceAnnals of microbiology 64, pp. 307–311
KeywordsTriazine herbicidesFluorescence in situ hybridizationSoil bacterial diversity
JournalAnnals of microbiology
EditorSpringer, Heidelberg, Germania
Year2014
TypeArticolo in rivista
DOI10.1007/s13213-013-0665-2
AuthorsGodoi I, Sene L, Barra Caracciolo A
Text225979 2014 10.1007/s13213 013 0665 2 Scopus 2 s2.0 84894431558 Triazine herbicides Fluorescence in situ hybridization Soil bacterial diversity Assessment of the bacterial community structure in a Brazilian clay soil treated with atrazine Godoi I, Sene L, Barra Caracciolo A State University of West Parana, Cascavel Brasile; Istituto di Ricerca Sulle Acque IRSA CNR In the present paper, the bacterial communities in two soils, one from an agricultural sugarcane cropped field and the other from an unperturbed soil with similar geopedological characteristics, were characterized using the Fluorescence In Situ Hybridization FISH method. FISH consists of in situ identification of bacteria using fluorescent labeled 16S rRNA targeted oligonucleotide probes visualizable under epifluorescence microscope. In the cultivated soil, in line with agricultural practice, the pre emergence herbicide atrazine had been regularly applied each year at a concentration of 5 L/ha. The Shannon Diversity and Evenness Indices were also calculated using the phylogenetic data obtained from the FISH analysis. Although, at the sampling time 6 months after soil atrazine treatment , no residual herbicide concentration was found, the overall bacterial community results show a lower diversity and evenness in the agricultural soil than in the unperturbed one, demonstrating how microbiological indicators are sensitive to anthropogenic disturbance. In the natural soil, the dominant groups were a Proteobacteria, Proteobacteria, and g Proteobacteria representing more than 50 % of the bacteria , but in the agricultural soil, their abundance decreased significantlyand represented just 31 % of the bacteria domain. 64 Published version Assessment of the bacterial community structure in a Brazilian clay soil treated with atrazine articolo pubblicato AnnalsofMicrobiology.pdf Articolo in rivista Springer 1590 4261 Annals of microbiology Annals of microbiology Ann. microbiol. Annals of microbiology. Annals of microbiology Print anna.barracaracciolo BARRA CARACCIOLO ANNA TA.P04.005.011 Vulnerabilita degli ecosistemi delle acque sotterranee e attenuazione naturale degli inquinanti nel suolo e nel sottosuolo