| Title | Neuroglial cells in long-term primary cultures from the gilthead seabream (Sparus aurata L.): new functional in vitro model from bony fish brain. |
| Abstract | As its Greek name suggests, neuroglia is the glue of the nervous system, simply referred to as non-neuronal cells, with supporting functions for neurons in the central nervous system (CNS) of mammalian and lower vertebrates. Recent advances in stem cell biology have redefined new functions. In fish, neuroglial cells have been described as supporting cells, and as such require further investigation. Approximately 283 cell lines have been obtained from fish worldwide, yet none from the brain of Sparus aurata, neither in cell lines nor as primary culture. We describe a novel reproducible in vitro neuroglial marine model for establishing primary neuroglial cell cultures by dissociating the whole brain of seabream juveniles. Proliferating neural stem cells produced alongside three generating lineages (neuronal precursor cells, astroglial precursor cells and oligodendroglia precursor cells) developed neurons, astrocytes and oligodendrocytes, respectively. The radial glia, finely described by morphological studies and immunochemical antigen expression, showed a particular spatial distribution, giving rise simultaneously both to astrocytes and neuronal precursors within a highly proliferative assemblage. Radial glial cells were assessed by glial fibrillary acidic protein (GFAP) and vimentin reactivity, astrocytes by GFAP, neurons by the neuron-specific markers for ubiquitin carboxy-terminal hydrolase 1 (UCHL1) and intermediate filament associated protein (NF). Myelinating oligodendrocytes were immunostained with anti-myelin basic protein (MBP) and anti-O4. Findings suggest that seabream neuroglial cells at 3-4 weeks of culture proliferation acquire neuroglial differentiation and oligodendrocyte maturation with myelination, suggesting that mixed neuroglial cultures accelerate maturation of oligodendrocytes and regenerate CNS injury in fish. |
| Source | Italian Journal of Animal Science (Online) 12 (1) |
| Keywords | Neuroglia; Astrocytes; Microglia; Radial glia; Sparus aurata |
| Journal | Italian Journal of Animal Science (Online) |
| Editor | PagePress Publications,, Pavia, Italia |
| Year | 2013 |
| Type | Articolo in rivista |
| Authors | Centoducati G., Zacchino V., Narracci M., Iaffaldano N., Santacroce M.P. |
| Text | 215405 2013 Neuroglia; Astrocytes; Microglia; Radial glia; Sparus aurata Neuroglial cells in long term primary cultures from the gilthead seabream Sparus aurata L. new functional in vitro model from bony fish brain. Centoducati G., Zacchino V., Narracci M., Iaffaldano N., Santacroce M.P. Dipartimento di Produzione Animale, Universita di Bari Aldo Moro, Italy Istituto per l Ambiente Marino Costiero, Consiglio Nazionale delle Ricerche, Taranto, Italy Dipartimento di Scienze Animali, Vegetali e Ambientali, Universita del Molise, Campobasso, Italy Dipartimento di Sanita Pubblica e Zootecnia, Universita di Bari Aldo Moro, Italy As its Greek name suggests, neuroglia is the glue of the nervous system, simply referred to as non neuronal cells, with supporting functions for neurons in the central nervous system CNS of mammalian and lower vertebrates. Recent advances in stem cell biology have redefined new functions. In fish, neuroglial cells have been described as supporting cells, and as such require further investigation. Approximately 283 cell lines have been obtained from fish worldwide, yet none from the brain of Sparus aurata, neither in cell lines nor as primary culture. We describe a novel reproducible in vitro neuroglial marine model for establishing primary neuroglial cell cultures by dissociating the whole brain of seabream juveniles. Proliferating neural stem cells produced alongside three generating lineages neuronal precursor cells, astroglial precursor cells and oligodendroglia precursor cells developed neurons, astrocytes and oligodendrocytes, respectively. The radial glia, finely described by morphological studies and immunochemical antigen expression, showed a particular spatial distribution, giving rise simultaneously both to astrocytes and neuronal precursors within a highly proliferative assemblage. Radial glial cells were assessed by glial fibrillary acidic protein GFAP and vimentin reactivity, astrocytes by GFAP, neurons by the neuron specific markers for ubiquitin carboxy terminal hydrolase 1 UCHL1 and intermediate filament associated protein NF . Myelinating oligodendrocytes were immunostained with anti myelin basic protein MBP and anti O4. Findings suggest that seabream neuroglial cells at 3 4 weeks of culture proliferation acquire neuroglial differentiation and oligodendrocyte maturation with myelination, suggesting that mixed neuroglial cultures accelerate maturation of oligodendrocytes and regenerate CNS injury in fish. 12 Published version Articolo in rivista PagePress Publications, 1828 051X Italian Journal of Animal Science Online Italian Journal of Animal Science Online Ital. J. Anim. Sci. Online Italian Journal of Animal Science. Online IJAS Online marcella.narracci NARRACCI MARCELLA |
