| Title | The lipidic extract of the seaweed Gracilariopsis longissima (Rhodophyta, Gracilariales): a potential resource for biotechnological purposes? |
| Abstract | In recent years seaweeds increasingly attracted interest in the search for new drugs and have been shown
to be a primary source of bioactive natural products including antibiotics. In the present investigation
the antimicrobial activity of Gracilariopsis longissima lipidic extract was assayed and its chemical
characterization was carried out by means of advanced analytical techniques such as gaschromatography
and multinuclear and multidimensional NMR spectroscopy. G. longissima lipidic
extract showed an antibacterial activity against several Vibrio species. These results are interesting
considering both the resistance against antibiotics developed by vibrios and the need to control fish and
shellfish diseases due to vibriosis. Analysis of fatty acid methyl esters performed by gas-chromatography
showed that palmitic acid methyl ester (16:0) was the predominant saturated fatty acid (42%), while,
among monounsaturated fatty acids, oleic acid methyl ester (18:1) prevailed (8.5%). Because the
palmitic acid represents the main component of fatty acids we hypothesized its involvement in the
antibacterial activity observed. However, a pure sample of palmitic acid did not show an antibacterial
activity. The fatty acid profile of G. longissima revealed also an interesting composition in
polyunsaturated fatty acids and in particular the ratio of v-3 to v-6 fatty acids was >1 thus suggesting
that this macroalga may be used as a natural source of v3. Moreover, the 1H NMR spectrum in CDCl3 of
algal lipid fraction shows the characteristic signals of saturated and unsaturated fatty acids as well as
other metabolites. Interestingly, in the lipid extract the presence of polyhydroxybutyrate, a linear
biodegradable and biocompatible polyester, was clearly identified by NMR spectroscopy. In conclusion,
the lipidic extract of G. longissima on account of its antimicrobial activity, nutritional value and
content in biodegradable and biocompatible polyester represents an interesting potential
biotechnological resource. |
| Source | New biotechnology (Print) 29 (3)–443 |
| Keywords | FATTY-ACID COMPOSITIONGRAM-POSITIVE BACTERIARED ALGAANTIBACTERIALAQUACULTURE |
| Journal | New biotechnology (Print) |
| Editor | Elsevier Life Science, Amsterdam, Paesi Bassi |
| Year | 2012 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.nbt.2011.11.003 |
| Authors | STABILI L., ACQUAVIVA M.I., BIANDOLINO F., CAVALLO R.A., DE PASCALI S.A., FANIZZI F., NARRACCI M., PETROCELL A., CECERE E. |
| Text | 215353 2012 10.1016/j.nbt.2011.11.003 ISI Web of Science WOS WOS 000301008800026 FATTY ACID COMPOSITION GRAM POSITIVE BACTERIA RED ALGA ANTIBACTERIAL AQUACULTURE The lipidic extract of the seaweed Gracilariopsis longissima Rhodophyta, Gracilariales a potential resource for biotechnological purposes STABILI L., ACQUAVIVA M.I., BIANDOLINO F., CAVALLO R.A., DE PASCALI S.A., FANIZZI F., NARRACCI M., PETROCELL A., CECERE E. IAMC CNR U.O.S. di Taranto DiSTeBA Universita del Salento In recent years seaweeds increasingly attracted interest in the search for new drugs and have been shown to be a primary source of bioactive natural products including antibiotics. In the present investigation the antimicrobial activity of Gracilariopsis longissima lipidic extract was assayed and its chemical characterization was carried out by means of advanced analytical techniques such as gaschromatography and multinuclear and multidimensional NMR spectroscopy. G. longissima lipidic extract showed an antibacterial activity against several Vibrio species. These results are interesting considering both the resistance against antibiotics developed by vibrios and the need to control fish and shellfish diseases due to vibriosis. Analysis of fatty acid methyl esters performed by gas chromatography showed that palmitic acid methyl ester 16 0 was the predominant saturated fatty acid 42% , while, among monounsaturated fatty acids, oleic acid methyl ester 18 1 prevailed 8.5% . Because the palmitic acid represents the main component of fatty acids we hypothesized its involvement in the antibacterial activity observed. However, a pure sample of palmitic acid did not show an antibacterial activity. The fatty acid profile of G. longissima revealed also an interesting composition in polyunsaturated fatty acids and in particular the ratio of v 3 to v 6 fatty acids was >1 thus suggesting that this macroalga may be used as a natural source of v3. Moreover, the 1H NMR spectrum in CDCl3 of algal lipid fraction shows the characteristic signals of saturated and unsaturated fatty acids as well as other metabolites. Interestingly, in the lipid extract the presence of polyhydroxybutyrate, a linear biodegradable and biocompatible polyester, was clearly identified by NMR spectroscopy. In conclusion, the lipidic extract of G. longissima on account of its antimicrobial activity, nutritional value and content in biodegradable and biocompatible polyester represents an interesting potential biotechnological resource. 29 Published version Articolo in rivista Elsevier Life Science 1871 6784 New biotechnology Print New biotechnology Print New biotechnology. Print antonella.petrocelli PETROCELLI ANTONELLA loredana.stabili STABILI LOREDANA mariaimmacolata.acquaviva ACQUAVIVA MARIA IMMACOLATA marcella.narracci NARRACCI MARCELLA francesca.biandolino BIANDOLINO FRANCESCA ester.cecere CECERE ESTER rosaanna.cavallo CAVALLO ROSA ANNA TA.P04.029.002 Utilizzazione di biomasse e sostanze attive da organismi marini biotecnologie per loo sviluppo di nuovi bio materiali e composti bioattivi |
