| Title | Metabolic model for the filamentous 'Candidatus Microthrix parvicella' based on genomic and metagenomic analyses |
| Abstract | Candidatus Microthrix parvicella' is a lipid-accumulating, filamentous bacterium so far found only in activated sludge wastewater treatment plants, where it is a common causative agent of sludge separation problems. Despite attracting considerable interest, its detailed physiology is still unclear. In this study, the genome of the RN1 strain was sequenced and annotated, which facilitated the construction of a theoretical metabolic model based on available in situ and axenic experimental data. This model proposes that under anaerobic conditions, this organism accumulates preferentially long-chain fatty acids as triacylglycerols. Utilisation of trehalose and/or polyphosphate stores or partial oxidation of long-chain fatty acids may supply the energy required for anaerobic lipid uptake and storage. Comparing the genome sequence of this isolate with metagenomes from two full-scale wastewater treatment plants with enhanced biological phosphorus removal reveals high similarity, with few metabolic differences between the axenic and the dominant community 'Ca. M. parvicella' strains. Hence, the metabolic model presented in this paper could be considered generally applicable to strains in full-scale treatment systems. The genomic information obtained here will provide the basis for future research into in situ gene expression and regulation. Such information will give substantial insight into the ecophysiology of this unusual and biotechnologically important filamentous bacterium. |
| Source | The ISME journal (Online) 7 (6), pp. 1161–1172 |
| Keywords | wastewater treatment; Microthrix parvicella; bulking and foaming; activated sludge |
| Journal | The ISME journal (Online) |
| Editor | Nature Publishing Group, London, Regno Unito |
| Year | 2013 |
| Type | Articolo in rivista |
| DOI | 10.1038/ismej.2013.6 |
| Authors | Jon McIlroy S, Kristiansen R, Albertsen M, Michael Karst S, Rossetti S, Lund Nielsen J, Tandoi V, James Seviour R, Nielsen PH. |
| Text | 209680 2013 10.1038/ismej.2013.6 wastewater treatment; Microthrix parvicella; bulking and foaming; activated sludge Metabolic model for the filamentous Candidatus Microthrix parvicella based on genomic and metagenomic analyses Jon McIlroy S, Kristiansen R, Albertsen M, Michael Karst S, Rossetti S, Lund Nielsen J, Tandoi V, James Seviour R, Nielsen PH. Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Aalborg, Denmark. Water Research Institute, IRSA CNR Via Salaria km 29,300 00015 Monterotondo RM Italy Candidatus Microthrix parvicella is a lipid accumulating, filamentous bacterium so far found only in activated sludge wastewater treatment plants, where it is a common causative agent of sludge separation problems. Despite attracting considerable interest, its detailed physiology is still unclear. In this study, the genome of the RN1 strain was sequenced and annotated, which facilitated the construction of a theoretical metabolic model based on available in situ and axenic experimental data. This model proposes that under anaerobic conditions, this organism accumulates preferentially long chain fatty acids as triacylglycerols. Utilisation of trehalose and/or polyphosphate stores or partial oxidation of long chain fatty acids may supply the energy required for anaerobic lipid uptake and storage. Comparing the genome sequence of this isolate with metagenomes from two full scale wastewater treatment plants with enhanced biological phosphorus removal reveals high similarity, with few metabolic differences between the axenic and the dominant community Ca. M. parvicella strains. Hence, the metabolic model presented in this paper could be considered generally applicable to strains in full scale treatment systems. The genomic information obtained here will provide the basis for future research into in situ gene expression and regulation. Such information will give substantial insight into the ecophysiology of this unusual and biotechnologically important filamentous bacterium. 7 Published version Metabolic model for the filamentous Candidatus Microthrix parvicella based on genomic and metagenomic analyses pdf della pubblicazione ismej20136a.pdf Articolo in rivista Nature Publishing Group 1751 7370 The ISME journal Online The ISME journal Online ISME j. Online The ISME journal Online International Society for Microbial Ecology journal Online simona.rossetti ROSSETTI SIMONA valter.tandoi TANDOI VALTER |
