| Title | Electrochemical stimulation of microbial cis-dichloroethene (cis-DCE) oxidation by an ethene-assimilating culture |
| Abstract | This study investigated the feasibility of using a polarized graphite electrode as direct or indirect (via electrolytic oxygen generation) electron acceptor to stimulate the microbial oxidation of cis-dichloroethene (cis-DCE) in contaminated groundwater. A microbial culture was enriched in the anode chamber of a bioelectrochemical cell using a mixture of cis-DCE and ethene as substrates. The bioelectrochemical cell was operated by controlling the anode potential at +1.0V and +1.5V vs. the standard hydrogen electrode (SHE). Enhanced cometabolic removal of cis-DCE, with ethene serving as the growth substrate, was observed in batch tests with the anode polarized at +1.5V vs. SHE. At this potential, (chloro)ethenes removal was likely sustained by molecular oxygen generated at the anode from water oxidation. Conversely, negligible anaerobic degradation was observed at +1.0V vs. SHE (a potential which does not allow oxygen generation), hence suggesting that molecular oxygen is needed to initiate (chloro)ethene degradation. PCR-DGGE analysis of the microbial culture followed by band sequencing and phylogenetic analysis evidenced the selective enrichment of a Bacillus species, proving a strong indication that this microorganism was responsible for cis-DCE and ethene degradation. |
| Source | New biotechnology (Online) 30 (6), pp. 749–755 |
| Journal | New biotechnology (Online) |
| Editor | Elsevier, Amsterdam, Paesi Bassi |
| Year | 2013 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.nbt.2013.04.003 |
| Authors | Aulenta F, Verdini R, Zeppilli M, Zanaroli G, Fava F, Rossetti S, Majone M |
| Text | 209679 2013 10.1016/j.nbt.2013.04.003 Electrochemical stimulation of microbial cis dichloroethene cis DCE oxidation by an ethene assimilating culture Aulenta F, Verdini R, Zeppilli M, Zanaroli G, Fava F, Rossetti S, Majone M Water Research Institute, National Research Council IRSA CNR , Via Salaria km 29.300, 00015 Monterotondo, RM, Italy Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy Department of Civil, Chemical, Environmental, and Materials Engineering, University of Bologna, Via Terracini 28, 40131 Bologna, Italy This study investigated the feasibility of using a polarized graphite electrode as direct or indirect via electrolytic oxygen generation electron acceptor to stimulate the microbial oxidation of cis dichloroethene cis DCE in contaminated groundwater. A microbial culture was enriched in the anode chamber of a bioelectrochemical cell using a mixture of cis DCE and ethene as substrates. The bioelectrochemical cell was operated by controlling the anode potential at 1.0V and 1.5V vs. the standard hydrogen electrode SHE . Enhanced cometabolic removal of cis DCE, with ethene serving as the growth substrate, was observed in batch tests with the anode polarized at 1.5V vs. SHE. At this potential, chloro ethenes removal was likely sustained by molecular oxygen generated at the anode from water oxidation. Conversely, negligible anaerobic degradation was observed at 1.0V vs. SHE a potential which does not allow oxygen generation , hence suggesting that molecular oxygen is needed to initiate chloro ethene degradation. PCR DGGE analysis of the microbial culture followed by band sequencing and phylogenetic analysis evidenced the selective enrichment of a Bacillus species, proving a strong indication that this microorganism was responsible for cis DCE and ethene degradation. 30 Articolo in rivista Elsevier 1876 4347 New biotechnology Online New biotechnology Online New biotechnology. Online simona.rossetti ROSSETTI SIMONA federico.aulenta AULENTA FEDERICO TA.P07.017.001 Caratterizzazione di biomasse per l ottimizzazione dei processi biologici |
