Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Microcolony formation from single-cell Synechococcus Strains: a protective strategy against UVR |
| Abstract | UVR have different effects on prokaryotic cells as, for instance, filamentation and aggregation in bacteria. We studied the effect of UVR on microcolony formation in two freshwater picocyanobacterial strains from different ribotypes (Group B and Group I) and pigment composition (phycoerythrin, PE-rich and phycocyanin, PC-rich). The cultures were photoacclimated at low (LL, 10 µmol m-2 s-1) and medium (ML, 100 µmol m-2 s-1) light for two months and then exposed to UVR (Q-Panel) for 7 days. We observed the higher percentage of cells in microcolonies in PE-rich strain acclimated to LL and then exposed to UVR. Conversely, PE acclimated to ML and PC did not aggregate significantly. We identified and measured the specific carotenoids by HPLC at the different times of the experiment. We suggest that the induction of protective pigments during ML acclimation enables single-cell survival during UVR irradiation and that the strategy of microcolony formation is a protective response of LL acclimated cells. The extent to which the microcolony formation from single cell Synechococcus is related to pigment type, phylogenetic position or photoacclimation is discussed. |
| Source | ASLO: Congress of the Association for the Sciences of Limnology and Oceanography. Aquatic Sciences Meeting, San Juan, Puerto Rico, 13-18 febbraio 2011 |
| Keywords | Microcolony formationSynechococcusUV effect |
| Year | 2011 |
| Type | Presentazione |
| Authors | Cristiana Callieri; Roberto Bertoni; Andrea Lami |
| Text | 205301 2011 Microcolony formation Synechococcus UV effect Microcolony formation from single cell Synechococcus Strains a protective strategy against UVR Cristiana Callieri; Roberto Bertoni; Andrea Lami CNR ISE, Pallanza Verbania UVR have different effects on prokaryotic cells as, for instance, filamentation and aggregation in bacteria. We studied the effect of UVR on microcolony formation in two freshwater picocyanobacterial strains from different ribotypes Group B and Group I and pigment composition phycoerythrin, PE rich and phycocyanin, PC rich . The cultures were photoacclimated at low LL, 10 µmol m 2 s 1 and medium ML, 100 µmol m 2 s 1 light for two months and then exposed to UVR Q Panel for 7 days. We observed the higher percentage of cells in microcolonies in PE rich strain acclimated to LL and then exposed to UVR. Conversely, PE acclimated to ML and PC did not aggregate significantly. We identified and measured the specific carotenoids by HPLC at the different times of the experiment. We suggest that the induction of protective pigments during ML acclimation enables single cell survival during UVR irradiation and that the strategy of microcolony formation is a protective response of LL acclimated cells. The extent to which the microcolony formation from single cell Synechococcus is related to pigment type, phylogenetic position or photoacclimation is discussed. Published version ID_PUMA /cnr.ise/2011 A3 014Area di valutazione04 Scienze della Terra ASLO Congress of the Association for the Sciences of Limnology and Oceanography. Aquatic Sciences Meeting San Juan, Puerto Rico 13 18 febbraio 2011 Nazionale Contributo PPT 2011_PPT_ASLO_Synechoccus.pdf Presentazione roberto.bertoni BERTONI ROBERTO cristiana.callieri CALLIERI CRISTIANA andrea.lami LAMI ANDREA TA.P04.016.004 Ecologia teorica e applicata degli ecosistemi acquatici |