Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Early release and subsequent caspase-mediated degradation of cytochrome c in apoptotic cerebellar granule cells. |
| Abstract | Cytochrome c (cyt c) release was investigated in cerebellar granule cells used as an in vitro neuronal model of apoptosis. We have found that cyt c is released into the cytoplasm as an intact, functionally active protein, that this event occurs early, in the commitment phase of the apoptotic process, and that after accumulation, this protein is progressively degraded. Degradation, but not release, is fully blocked by benzyloxycarbonyl-Val-Ala-Asp-fluoromethylchetone (z-VAD-fmk). On the basis of previous findings obtained in the same neuronal population undergoing excitotoxic death, it is hypothesized that release of cyt c may be part of a cellular attempt to maintain production of ATP via cytochrome oxidase, which is reduced by cytosolic NADH in a cytochrome b5-soluble cyt c-mediated fashion. |
| Source | FEBS letters (Print) 457, pp. 126–130 |
| Journal | FEBS letters (Print) |
| Editor | Elsevier Science Publishers, Amsterdam, Paesi Bassi |
| Year | 1999 |
| Type | Articolo in rivista |
| Authors | Bobba A, Atlante A, Giannattasio S, Sgaramella G, Calissano P, Marra E |
| Text | 203190 1999 PubMed 10486578 Early release and subsequent caspase mediated degradation of cytochrome c in apoptotic cerebellar granule cells. Bobba A, Atlante A, Giannattasio S, Sgaramella G, Calissano P, Marra E Istituto di Biomembrane e Bioenergetica, CNR Bari Cytochrome c cyt c release was investigated in cerebellar granule cells used as an in vitro neuronal model of apoptosis. We have found that cyt c is released into the cytoplasm as an intact, functionally active protein, that this event occurs early, in the commitment phase of the apoptotic process, and that after accumulation, this protein is progressively degraded. Degradation, but not release, is fully blocked by benzyloxycarbonyl Val Ala Asp fluoromethylchetone z VAD fmk . On the basis of previous findings obtained in the same neuronal population undergoing excitotoxic death, it is hypothesized that release of cyt c may be part of a cellular attempt to maintain production of ATP via cytochrome oxidase, which is reduced by cytosolic NADH in a cytochrome b5 soluble cyt c mediated fashion. 457 Early release and subsequent caspase mediated degradation of cytochrome c in apoptotic cerebellar granule cells early_cyt_c_1999.pdf Articolo in rivista Elsevier Science Publishers 0014 5793 FEBS letters Print FEBS letters Print FEBS lett. Print FEBS letters. Print Federation of European Biochemical Societies letters Print anna.atlante ATLANTE ANNA antonella.bobba BOBBA ANTONELLA giuseppe.sgaramella SGARAMELLA GIUSEPPE sergio.giannattasio GIANNATTASIO SERGIO SV.P15.003.001 Interrelazione nucleo/citoplasma/mitocondri nell omeostasi cellulare. |