| Title | Isolation of Squarebop I bacteriorhodopsin from biomass of coastal salterns |
| Abstract | Squarebop I bacteriorhodopsin is a light-activated proton pump present in the membranes of the archeon
Haloquadratum walsbyi, a square-shaped organism representing 50-60% of microbial population in the
crystallizer ponds of the coastal salterns.
Here we describe: (1) the operating mode of a bioreactor designed to concentrate the saltern biomass
through a microfiltration process based on polyethersulfone hollow fibers; (2) the isolation of Squarebop
I bacteriorhodopsin from solubilized biomass by means of a single chromatographic step; (3) tightly
bound lipids to the isolated and purified protein as revealed by MALDI-TOF/MS analysis; (4) the photoactivity
of Squarebop I bacteriorhodopsin isolated from environmental samples by flash spectroscopy.
Yield of the isolation process is 150 lg of Squarebop I bacteriorhodopsin from 1 l of 25-fold concentrated
biomass. The possibility of using the concentrated biomass of salterns, as renewable resource
for the isolation of functional bacteriorhodopsin and possibly other valuable bioproducts, is briefly
discussed. |
| Source | Protein expression and purification (Print) 84, pp. 73–79 |
| Keywords | Retinal proteinsMicrofiltrationHollow fibersHalophilic archaea |
| Journal | Protein expression and purification (Print) |
| Editor | Academic Press,, San Diego, Stati Uniti d'America |
| Year | 2012 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.pep.2012.04.017 |
| Authors | Lobasso S.(a), Lopalco P.(b), Angelini R.(a), Pollice A.(c), Laera G.(c), Milano F.(d), Agostiano A.(d,e), Corcelli A. (a,d) |
| Text | 191953 2012 10.1016/j.pep.2012.04.017 ISI Web of Science WOS 000305779000009 Retinal proteins Microfiltration Hollow fibers Halophilic archaea Isolation of Squarebop I bacteriorhodopsin from biomass of coastal salterns Lobasso S. a , Lopalco P. b , Angelini R. a , Pollice A. c , Laera G. c , Milano F. d , Agostiano A. d,e , Corcelli A. a,d a Department of Basic Medical Sciences, University of Bari Aldo Moro , Bari, Italy b Institute of Microelectronics and Microsystems National Research Council, IMM CNR, Lecce, Italy c Water Research Institute National Research Council, IRSA CNR, Bari, Italy d Institute for Chemical Physical Processes National Research Council, IPCF CNR, Bari, Italy e Department of Chemistry, University of Bari Aldo Moro , Bari, Italy Squarebop I bacteriorhodopsin is a light activated proton pump present in the membranes of the archeon Haloquadratum walsbyi, a square shaped organism representing 50 60% of microbial population in the crystallizer ponds of the coastal salterns. Here we describe 1 the operating mode of a bioreactor designed to concentrate the saltern biomass through a microfiltration process based on polyethersulfone hollow fibers; 2 the isolation of Squarebop I bacteriorhodopsin from solubilized biomass by means of a single chromatographic step; 3 tightly bound lipids to the isolated and purified protein as revealed by MALDI TOF/MS analysis; 4 the photoactivity of Squarebop I bacteriorhodopsin isolated from environmental samples by flash spectroscopy. Yield of the isolation process is 150 lg of Squarebop I bacteriorhodopsin from 1 l of 25 fold concentrated biomass. The possibility of using the concentrated biomass of salterns, as renewable resource for the isolation of functional bacteriorhodopsin and possibly other valuable bioproducts, is briefly discussed. 84 Published version Isolation of Squarebop I bacteriorhodopsin from biomass of coastal salterns 1_s2_0_S1046592812001258_main.pdf Articolo in rivista Academic Press, 1046 5928 Protein expression and purification Print Protein expression and purification Print Protein expr. purif. Print Protein expression and purification. Print Protein expression purification Print angelacorcelli CORCELLI ANGELA angela.agostiano AGOSTIANO ANGELA alfieri.pollice POLLICE ALFIERI giuseppe.laera LAERA GIUSEPPE francesco.milano MILANO FRANCESCO patrizia.lopalco LOPALCO PATRIZIA TA.P07.002.004 Processi biotecnologici e processi a membrana |
