| Text | 188013 2012 10.1002/etc.1909 Acartia tonsa Acute test Semichronic test Standardized methods Interlaboratory test STANDARDIZED METHODS FOR ACUTE AND SEMICHRONIC TOXICITY GESSICA GORBI;MARION INVIDIA; FEDERICA SAVORELLI; OLGA FARAPONOVA; ELISABETTA GIACCO; MONICA CIGAR; ISABELLA BUTTINO; TRISTANO LEONI;ERMELINDA PRATO;INES LACCHETTI; SANDRA SEI Department of Environmental Sciences, University of Parma, Parma, Italy Integrated Laboratory, Regional Agency for Environmental Protection, Ferrara Division, Ferrara, Italy Institute for Environmental Protection and Research, Rome, Italy Department for the Study of the Territory and its Resources, University of Genoa, Genoa, Italy Shoreline, Trieste, Italy Institute for Environmental Protection and Research, Livorno, Italy, and the Anton Dohrn Zoological Station, Naples, Italy Regional Agency for Environmental Protection, Macerata Department, Macerata, Italy Institute for the Coastal Marine Environment, National Research Council, Taranto, Italy Institute of Health, Department of the Environment and Primary Prevention, Soil and Waste Unit, Ecotoxicology Laboratory, Rome, Italy The availability of standardized protocols for both organism culture and bioassay with ecologically relevant species is of great concern in ecotoxicology. Acartia tonsa represents an important, often dominant, member of zooplankton communities and meets all the practical criteria suggested for model species. New standardized procedures for laboratory culturing of the copepod A. tonsa and standardized methods for acute 24 and 48 h and semichronic 7 d, static renewal toxicity tests with the nauplius stage are described. In both cases, eggs are the starting stage, and nauplius immobilization is the endpoint. The methods were the object of an intercomparison test involving nine laboratories, and nickel was the reference toxicant. Relative reproducibility was 24, 25, and 34% for 24 h, 48 h, and 7 d tests, respectively. 31 Articolo in rivista Elsevier Science 1552 8618 Environmental toxicology and chemistry Online Environmental toxicology and chemistry Online Environ. toxicol. chem. Online Environmental toxicology and chemistry Online ET C Online ET and C Online ermelinda.prato PRATO ERMELINDA |
