Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Q-RT-PCR Detection of substrate-specific gene expression |
| Keywords | Biofuels - Bioremediation - Biosynthesis and Degradation - Ecophysiology - Experimental Protocols - Food Microbiology - Microbes - Oil - Petroleum - Toxicology |
| Year | 2010 |
| Type | Contributo in volume |
| DOI | 10.1007/978-3-540-77587-4_199 |
| Authors | Denaro R; Yakimov M.M.; Genovese M.; |
| Text | 175136 2010 10.1007/978 3 540 77587 4_199 Biofuels Bioremediation Biosynthesis and Degradation Ecophysiology Experimental Protocols Food Microbiology Microbes Oil Petroleum Toxicology Q RT PCR Detection of substrate specific gene expression Denaro R; Yakimov M.M.; Genovese M.; Istituto per Ambiente marino costiero This Handbook is an essential reference to a topic of great current concern and interest Four volume reference offers exhaustive coverage The definitive resource of current knowledge on the diverse and multifaceted aspects of hydrocarbon and lipid microbiology Water is life All active cellular systems require water as the medium and solvent of their metabolic activities. Hydrophobic compounds and structures, which tend to exclude water, though providing inter alia excellent sources of energy and a means of biological compartmentalization, present problems of cellular handling, poor bioavailability and, in some cases, toxicity. Microbes both synthesize and exploit a vast range of hydrophobic organics, especially petroleum oil hydrocarbons and industrial pollutants, and the underlying interactions not only have major consequences for the lifestyles of the microbes involved, but also for biogeochemistry, climate change, environmental pollution, human health and a range of biotechnological applications. The aim of this handbook is to be the definitive resource of current knowledge on the diverse and multifaceted aspects of these interactions, the microbial players, and the physiological mechanisms and adaptive strategies characteristic of the microbial lifestyle that plays out at hydrophobic material aqueous liquid interfaces. The assessment of oil impact in marine environment must take into account two important issues 1 to establish the type or types of contaminants and 2 verify their bioavailability and transformation by microbial degradation. This chapter discuses the specialization of hydrocarbonoclastic bacteria, in particular Alcanivorax borkumensis SK2, in the selection of metabolic pathway to consume different substrates. The study is focused on the two major systems involved in alkanes degradation, cytochrome P450 and alkane hydrolases alkB and the related genes expression in the presence of two classes of hydrocarbons phytane naturally occurring isoprenoids and n tetradecane anthropogenic aliphatics . Results of Q RT PCR analysis of alkane hydrolases and cytochromes P450 expression in A. borkumensis revealed drastic changes at early exponential phase compared with those at late exponential phase of growth on tetradecane, for both genes studied, while a different behavior of alkB genes expression in isoprenoid growing cells was registered with no alkB1 mRNA detection and overexpression of alkB2 and P450 genes at early exponential phase of growth. Moreover, P450 c showed a significant expression in pristine growing cells and a house keeping type expression in phytane growing cells. Remarkably, phytane seems to be a strong inducer of cytochrome P450a gene expression and the highest copy number of its corresponding mRNA 20 ± 3 molecules per cell was detected. Handobook of Hydrocarbon and lipid microbiology 978 3 540 77584 3 Q RT PCR Detection of Substrate Specific Gene expression fulltext.pdf Contributo in volume mikhail.iakimov IAKIMOV MIKHAIL renata.denaro DENARO RENATA maria.genovese GENOVESE MARIA TA.P04.026.002 Impatto Antropogenico su ecosistemi marini microbiologia ed biotecnologia ambientale |