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TitleBioremediation of benzene, toluene, ethylbenzene, xylenes-contaminated soil: A biopile pilot experiment
AbstractAims: In this study, we evaluated the removal efficiency of fuel hydrocarbons from a jet fuel contaminated area using bioaugmentation treatment in biopile. Methods and Results: The hydrocarbon analysis of the sample revealed total hydrocarbons mainly constituted by benzene, toluene, ethylbenzene, xylenes (BTEX) and heavy aliphatic hydrocarbons. Enrichments of soil sample were performed with BTEX, pristane and fuel JP-5, respectively, selected hydrocarbon-degrading strains, namely Acinetobacter sp., Pseudomonas sp. and Rhodococcus sp. Three hundred litres of culture containing 10 8 cell ml -1 of each strain and nutrients sprayed on the biopile allowed a removal of 90% of total hydrocarbons in 15 days. Bioremediation process was monitored by observation of the respiration rate and the bacterial abundance and GC-MS analysis. Conclusions: The efficiency of the treatment in the biopile was considerable. The assessment of microbial activity during the experiment is necessary for interventions targeted to improve environmental parameters such as humidity, temperature, pH and nutrients for optimization of the bioremediation process. Significance and Impact of the Study: A better knowledge of microbial successions at oil-polluted sites is essential for environmental bioremediation. Data obtained in biopile study improve our understanding of processes occurring during oil pollution.
SourceJournal of applied microbiology (Print) 105 (5), pp. 1694–1702
KeywordsBioaugmentation; Biopile; Bioremediation; BTEX; Hydrocarbon-degrading bacteria
JournalJournal of applied microbiology (Print)
EditorBlackwell Science., Oxford, Regno Unito
Year2008
TypeArticolo in rivista
DOI10.1111/j.1365-2672.2008.03897.x
AuthorsGenovese, M.; Denaro, R.; Cappello, S.; Di Marco, G.; La Spada, G.; Giuliano, L.; Genovese, L.; Yakimov, M.M.;
Text175099 2008 10.1111/j.1365 2672.2008.03897.x Scopus 2 s2.0 54049146297 ISI Web of Science WOS 000260113200050 Bioaugmentation; Biopile; Bioremediation; BTEX; Hydrocarbon degrading bacteria Bioremediation of benzene, toluene, ethylbenzene, xylenes contaminated soil A biopile pilot experiment Genovese, M.; Denaro, R.; Cappello, S.; Di Marco, G.; La Spada, G.; Giuliano, L.; Genovese, L.; Yakimov, M.M.; Istituto Per l Ambiente Marino Costiero IAMC IST CNR Section of Messina, Messina, Italy Istituto Per I Processi Chimico fisici IPCF CNR of Messina, Messina, Italy Aims In this study, we evaluated the removal efficiency of fuel hydrocarbons from a jet fuel contaminated area using bioaugmentation treatment in biopile. Methods and Results The hydrocarbon analysis of the sample revealed total hydrocarbons mainly constituted by benzene, toluene, ethylbenzene, xylenes BTEX and heavy aliphatic hydrocarbons. Enrichments of soil sample were performed with BTEX, pristane and fuel JP 5, respectively, selected hydrocarbon degrading strains, namely Acinetobacter sp., Pseudomonas sp. and Rhodococcus sp. Three hundred litres of culture containing 10 8 cell ml 1 of each strain and nutrients sprayed on the biopile allowed a removal of 90% of total hydrocarbons in 15 days. Bioremediation process was monitored by observation of the respiration rate and the bacterial abundance and GC MS analysis. Conclusions The efficiency of the treatment in the biopile was considerable. The assessment of microbial activity during the experiment is necessary for interventions targeted to improve environmental parameters such as humidity, temperature, pH and nutrients for optimization of the bioremediation process. Significance and Impact of the Study A better knowledge of microbial successions at oil polluted sites is essential for environmental bioremediation. Data obtained in biopile study improve our understanding of processes occurring during oil pollution. 105 Bioremediation of benzene, toluene, ethylbenzene, xylenes contaminated soil A biopile pilot experiment j_1365_2672_2008_03897_x.pdf Articolo in rivista Blackwell Science. 1364 5072 Journal of applied microbiology Print Journal of applied microbiology Print J. appl. microbiol. Print LA SPADA GINA gaetano.dimarco DI MARCO GAETANO lucrezia.genovese GENOVESE LUCREZIA laura.giuliano GIULIANO LAURA mikhail.iakimov IAKIMOV MIKHAIL renata.denaro DENARO RENATA simone.cappello CAPPELLO SIMONE maria.genovese GENOVESE MARIA MD.P01.014.005 Dispositivi fotoelettrochimici, polimeri e termodinamica dei processi irreversibili TA.P04.026.002 Impatto Antropogenico su ecosistemi marini microbiologia ed biotecnologia ambientale