| Title | Thalassolituus oleivorans gen. nov., sp. nov., a novel marine bacterium that obligately utilizes hydrocarbons |
| Abstract | An aerobic, heterotrophic, Gram-negative, curved bacterial strain, designated MIL-1T, was isolated by extinction dilution from an n-tetradecane enrichment culture that was established from sea water/sediment samples collected in the harbour of Milazzo, Italy. In the primary enrichment, the isolate formed creamy-white, medium-sized colonies on the surface of the agar. The isolate did not grow in the absence of NaCl; growth was optimal at 2.7 % NaCl. Only a narrow spectrum of organic compounds, including aliphatic hydrocarbons (C7-C20), their oxidized derivatives and acetate, were used as growth substrates. The isolate was not able to grow under denitrifying conditions. The DNA G+C content and genome size of strain MIL-1Twere estimated to be 53.2 mol% and 2.2 Mbp, respectively. The major cellular and phospholipid fatty acids were palmitoleic, palmitic and oleic acids (33.5, 29.5 and 11.0 % and 18, 32 and 31 %, respectively). 3-Hydroxy lauric acid was the only hydroxy fatty acid detected. Thirteen different compounds that belonged to two types of phospholipid (phosphatidylethylamine and phosphatidylglycerol) were identified. 16S rRNA gene sequence analysis revealed that this isolate represents a distinct phyletic lineage within the ?-Proteobacteria and has about 94.4 % sequence similarity to Oceanobacter kriegii (the closest bacterial species with a validly published name). The deduced protein sequence of the putative alkane hydrolase, AlkB, of strain MIL-1T is related to the corresponding enzymes of Alcanivorax borkumensis and Pseudomonas oleovorans (81 and 80 % similarity, respectively). On the basis of the analyses performed, Thalassolituus oleivorans gen. nov., sp. nov. is described. Strain MIL-1T (=DSM 14913T=LMG 21420T) is the type and only strain of T. oleivorans. |
| Source | International journal of systematic and evolutionary microbiology (Print) 54 (1), pp. 141–148 |
| Keywords | Biodegradationmarine environmentalDNAHydrocarbonsbacterium identification |
| Journal | International journal of systematic and evolutionary microbiology (Print) |
| Editor | Society for General Microbiology., Reading, Regno Unito |
| Year | 2004 |
| Type | Articolo in rivista |
| DOI | 10.1099/ijs.0.02424-0 |
| Authors | Yakimov, M.M.a ; Giuliano, L.a; Denaro, R.a; Crisafi, E.a; Chernikova, T.N.b; Abraham, W.-R.b; Luensdorf, H.b; Timmis, K.N.b; Golyshin, P.N.b |
| Text | 174805 2004 10.1099/ijs.0.02424 0 Scopus 2 s2.0 0442326354 ISI Web of Science WOS 000189154700019 Biodegradation marine environmental DNA Hydrocarbons bacterium identification Thalassolituus oleivorans gen. nov., sp. nov., a novel marine bacterium that obligately utilizes hydrocarbons Yakimov, M.M.a ; Giuliano, L.a; Denaro, R.a; Crisafi, E.a; Chernikova, T.N.b; Abraham, W. R.b; Luensdorf, H.b; Timmis, K.N.b; Golyshin, P.N.b a Inst. per l Ambiente Marino Costiero, CNR Consiglio Nazionale Ricerche, Spianata San Raineri 86, 98122 Messina, Italy b Division of Microbiology, GBF National Res. Centre Biotechnol., Mascheroder Weg 1, 38124 Braunschweig, Germany An aerobic, heterotrophic, Gram negative, curved bacterial strain, designated MIL 1T, was isolated by extinction dilution from an n tetradecane enrichment culture that was established from sea water/sediment samples collected in the harbour of Milazzo, Italy. In the primary enrichment, the isolate formed creamy white, medium sized colonies on the surface of the agar. The isolate did not grow in the absence of NaCl; growth was optimal at 2.7 % NaCl. Only a narrow spectrum of organic compounds, including aliphatic hydrocarbons C7 C20 , their oxidized derivatives and acetate, were used as growth substrates. The isolate was not able to grow under denitrifying conditions. The DNA G C content and genome size of strain MIL 1Twere estimated to be 53.2 mol% and 2.2 Mbp, respectively. The major cellular and phospholipid fatty acids were palmitoleic, palmitic and oleic acids 33.5, 29.5 and 11.0 % and 18, 32 and 31 %, respectively . 3 Hydroxy lauric acid was the only hydroxy fatty acid detected. Thirteen different compounds that belonged to two types of phospholipid phosphatidylethylamine and phosphatidylglycerol were identified. 16S rRNA gene sequence analysis revealed that this isolate represents a distinct phyletic lineage within the Proteobacteria and has about 94.4 % sequence similarity to Oceanobacter kriegii the closest bacterial species with a validly published name . The deduced protein sequence of the putative alkane hydrolase, AlkB, of strain MIL 1T is related to the corresponding enzymes of Alcanivorax borkumensis and Pseudomonas oleovorans 81 and 80 % similarity, respectively . On the basis of the analyses performed, Thalassolituus oleivorans gen. nov., sp. nov. is described. Strain MIL 1T =DSM 14913T=LMG 21420T is the type and only strain of T. oleivorans. 54 Thalassolituus oleivorans gen. nov., sp. nov., a novel marine bacterium that obligately utilizes hydrocarbons Yakimov_M_M_2004_Int_J_Syst_Evol_Microbiol.pdf Articolo in rivista Society for General Microbiology. 1466 5026 International journal of systematic and evolutionary microbiology Print International journal of systematic and evolutionary microbiology Print Int. j. syst. evol. microbiol. Print IJSEM Print Print laura.giuliano GIULIANO LAURA mikhail.iakimov IAKIMOV MIKHAIL renata.denaro DENARO RENATA ermanno.crisafi CRISAFI ERMANNO |
