Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Detection of herbicide subclasses by an optical multibiosensor based on an array of Photosystem II mutants |
| Abstract | Massive use of herbicides in agriculture over the last few decades has become a serious environmental problem. The residual concentration of these compounds frequently exceeds the maximum admissible concentration in drinking water for human consumption and is a real environmental risk for the aquatic ecosystem. Herbicides inhibiting photosynthesis via targeting photosystem II function still represent the basic means of weed control. A multibiosensor was constructed for detecting herbicides using as biosensing elements photosynthetic preparations coupled to an optical fluorescence transduction system (Giardi et al. EU patent EP1134585, 01830148.1-2204); this paper is about its application in the detection of herbicide subclasses in river water. Photosynthetic material was immobilized on a silicio septum inside a series of flow cells, close to diodes so as to activate photosystem II (PSII) fluorescence. The principle of the detection was based on the fact that herbicides selectively modify PSII fluorescence activity. The multibiosensor has the original feature of being able to distinguish the subclasses of the photosynthetic herbicides by using specific immobilized biomediators isolated from mutated organisms. This setup resulted in a reusable, portable multibiosensor for the detection of herbicide subclasses with a half-life of 54 h for spinach thylakoids and limit of detection of 3x10-9M for herbicides present in river water. |
| Source | Environmental science & technology 39, pp. 5378–5384 |
| Journal | Environmental science & technology |
| Editor | American Chemical Society,, Easton, Pa., Stati Uniti d'America |
| Year | 2005 |
| Type | Articolo in rivista |
| DOI | 10.1021/es040511b |
| Authors | Giardi M. T., Guzzella L., Euzet P., Rouillon R., Esposito D. |
| Text | 170944 2005 10.1021/es040511b ISI Web of Science WOS 000230536200040 Detection of herbicide subclasses by an optical multibiosensor based on an array of Photosystem II mutants Giardi M. T., Guzzella L., Euzet P., Rouillon R., Esposito D. Giardi M. T.,Esposito D., CNR Istituto di Cristallografia, Roma Guzzella L., CNR IRSA, Brugherio MI Euzet P., Rouillon R., Universita di Perpignan, Francia Massive use of herbicides in agriculture over the last few decades has become a serious environmental problem. The residual concentration of these compounds frequently exceeds the maximum admissible concentration in drinking water for human consumption and is a real environmental risk for the aquatic ecosystem. Herbicides inhibiting photosynthesis via targeting photosystem II function still represent the basic means of weed control. A multibiosensor was constructed for detecting herbicides using as biosensing elements photosynthetic preparations coupled to an optical fluorescence transduction system Giardi et al. EU patent EP1134585, 01830148.1 2204 ; this paper is about its application in the detection of herbicide subclasses in river water. Photosynthetic material was immobilized on a silicio septum inside a series of flow cells, close to diodes so as to activate photosystem II PSII fluorescence. The principle of the detection was based on the fact that herbicides selectively modify PSII fluorescence activity. The multibiosensor has the original feature of being able to distinguish the subclasses of the photosynthetic herbicides by using specific immobilized biomediators isolated from mutated organisms. This setup resulted in a reusable, portable multibiosensor for the detection of herbicide subclasses with a half life of 54 h for spinach thylakoids and limit of detection of 3x10 9M for herbicides present in river water. 39 articolo Giardi_et_al.pdf Articolo in rivista American Chemical Society, 0013 936X Environmental science technology Environmental science technology Environ. sci. technol. Environmental science technology. ES T Environmental science and technology mariateresa.giardi GIARDI MARIA TERESA liciamaria.guzzella GUZZELLA LICIA MARIA TA.P04.016.001 Caratteristiche ecologiche di comunita acquatiche in ambienti fluviali e lagunari AG.P05.014.001 NUTRACEUTICI IN APPLICAZIONI SPAZIALI E TRASFERIMENTO TECNOLOGICO A TERRA |