| Title | NOVEL SULFONOLIPID IN THE EXTREMELY HALOPHILIC BACTERIUM SALINIBACTER RUBER |
| Abstract | Salinibacter ruber is an extremely halophilic bacterium, phylogenetically affiliated with the Flavobacterium/
Cytophaga branch of the domain Bacteria. Electrospray mass analyses (negative ion) of the total lipid extract
of a pure culture of S. ruber shows a characteristic peak at m/z 660 as the most prominent peak in the high-mass
range of the spectrum. A novel sulfonolipid, giving rise to the molecular ion [M-H] of m/z 660, has been
identified. The sulfonolipid isolated and purified by thin-layer chromatography was shown by chemical
degradation, mass spectrometry, infrared spectroscopy, and nuclear magnetic resonance analysis to have the
structure 2-carboxy-2-amino-3-O-(13-methyltetradecanoyl)-4-hydroxy-18-methylnonadec-5-ene-1-sulfonic acid.
This lipid represents about 10% of total cellular lipids, and it appears to be a structural variant of the sulfonolipids
found as main components of the cell envelope of gliding bacteria of the genus Cytophaga and closely related genera
(W. Godchaux and E. R. Leadbetter, J. Bacteriol. 153:1238-1246, 1983) and of diatoms (R. Anderson, M. Kates, and
B. E. Volcani, Biochim. Biophys. Acta 528:89-106, 1978). Since this sulfonolipid has never been observed in any other
extreme halophilic microorganism, we consider the peak at m/z 660 the lipid signature of Salinibacter. This study
suggests that this novel sulfonolipid may be used as a chemotaxonomic marker for the detection of Salinibacter
within the halophilic microbial community in saltern crystallizer ponds and other hypersaline environments. |
| Source | Applied and environmental microbiology (Print) 70 (11), pp. 6678–6685 |
| Journal | Applied and environmental microbiology (Print) |
| Editor | American Society for Microbiology., [Washington], Stati Uniti d'America |
| Year | 2004 |
| Type | Articolo in rivista |
| DOI | 10.1128/AEM.70.11.6678-6685.2004 |
| Authors | CORCELLI A., LATTANZIO V.M.T., MASCOLO G., BABUDRI F., OREN A., KATES M. |
| Text | 170221 2004 10.1128/AEM.70.11.6678 6685.2004 NOVEL SULFONOLIPID IN THE EXTREMELY HALOPHILIC BACTERIUM SALINIBACTER RUBER CORCELLI A., LATTANZIO V.M.T., MASCOLO G., BABUDRI F., OREN A., KATES M. Dipartimento di Biochimica Medica e Biologia Medica1 and Dipartimento di Chimica,4 Universita` degli Studi di Bari, IPCF CNR,2 IRSA CNR,3 and ICCOM CNR,5 Bari, Italy; The Institute of Life Sciences and The Moshe Shilo Minerva Center for Marine Biogeochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel6; and Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, Canada7 Salinibacter ruber is an extremely halophilic bacterium, phylogenetically affiliated with the Flavobacterium/ Cytophaga branch of the domain Bacteria. Electrospray mass analyses negative ion of the total lipid extract of a pure culture of S. ruber shows a characteristic peak at m/z 660 as the most prominent peak in the high mass range of the spectrum. A novel sulfonolipid, giving rise to the molecular ion M H of m/z 660, has been identified. The sulfonolipid isolated and purified by thin layer chromatography was shown by chemical degradation, mass spectrometry, infrared spectroscopy, and nuclear magnetic resonance analysis to have the structure 2 carboxy 2 amino 3 O 13 methyltetradecanoyl 4 hydroxy 18 methylnonadec 5 ene 1 sulfonic acid. This lipid represents about 10% of total cellular lipids, and it appears to be a structural variant of the sulfonolipids found as main components of the cell envelope of gliding bacteria of the genus Cytophaga and closely related genera W. Godchaux and E. R. Leadbetter, J. Bacteriol. 153 1238 1246, 1983 and of diatoms R. Anderson, M. Kates, and B. E. Volcani, Biochim. Biophys. Acta 528 89 106, 1978 . Since this sulfonolipid has never been observed in any other extreme halophilic microorganism, we consider the peak at m/z 660 the lipid signature of Salinibacter. This study suggests that this novel sulfonolipid may be used as a chemotaxonomic marker for the detection of Salinibacter within the halophilic microbial community in saltern crystallizer ponds and other hypersaline environments. 70 Novel Sulfonolipid in the Extremely Halophilic Bacterium Salinibacter ruber 2004_con_Corcelli_.pdf Articolo in rivista American Society for Microbiology. 0099 2240 Applied and environmental microbiology Print Applied and environmental microbiology Print Appl. environ. microbiol. Print Applied and environmental microbiology. Print angelacorcelli CORCELLI ANGELA veronicamariateresa.lattanzio LATTANZIO VERONICA MARIA TERESA giuseppe.mascolo MASCOLO GIUSEPPE MD.P01.011.001 Strutture ad alta organizzazione gerarchica realizzate mediante approcci di tipo biologico e chimico, per lo studio e la progettazione di materiali e sistemi ibridi di interesse fotochimico |
