Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Lipid-protein stoichiometries in a crystalline biological membrane: NMR quantitative analysis of the lipid extract of the purple membrane. |
| Abstract | The lipid/protein stoichiometries of a naturally crystalline biological membrane, the purple membrane (PM) of Halobacterium salinarum, have been obtained by a combination of (31)P- and (1)H-NMR analyses of the lipid extract. In total, 10 lipid molecules per retinal were found to be present in the PM lipid extract: 2-3 molecules of phosphatidylglycerophosphate methyl ester (PGP-Me), 3 of glycolipid sulfate, 1 of phosphatidylglycerol, 1 of archaeal glycocardiolipin (GlyC), 2 of squalene plus minor amounts of phosphatidylglycerosulfate (PGS) and bisphosphatidylglycerol (archaeal cardiolipin) (BPG) and a negligible amount of vitamin MK8.The novel data of the present study are necessary to identify the lipids in the electron density map, and to shed light on the structural relationships of the lipid and protein components of the PM. |
| Source | Journal of lipid research (Print) 43, pp. 132–140 |
| Journal | Journal of lipid research (Print) |
| Editor | American Society for Biochemistry and Molecular Biology, Bethesda, Md.,, Stati Uniti d'America |
| Year | 2002 |
| Type | Articolo in rivista |
| Authors | Corcelli A., Lattanzio V.M.T., Mascolo G., Papadia P., Fanizzi F. |
| Text | 168773 2002 Lipid protein stoichiometries in a crystalline biological membrane NMR quantitative analysis of the lipid extract of the purple membrane. Corcelli A., Lattanzio V.M.T., Mascolo G., Papadia P., Fanizzi F. Dipartimento di Fisiologia Generale ed Ambientale, Universita di Bari, CNR IRSA The lipid/protein stoichiometries of a naturally crystalline biological membrane, the purple membrane PM of Halobacterium salinarum, have been obtained by a combination of 31 P and 1 H NMR analyses of the lipid extract. In total, 10 lipid molecules per retinal were found to be present in the PM lipid extract 2 3 molecules of phosphatidylglycerophosphate methyl ester PGP Me , 3 of glycolipid sulfate, 1 of phosphatidylglycerol, 1 of archaeal glycocardiolipin GlyC , 2 of squalene plus minor amounts of phosphatidylglycerosulfate PGS and bisphosphatidylglycerol archaeal cardiolipin BPG and a negligible amount of vitamin MK8.The novel data of the present study are necessary to identify the lipids in the electron density map, and to shed light on the structural relationships of the lipid and protein components of the PM. 43 Pubblicazione internazionale Corcelli A., Lattanzio V. M. T., Mascolo G., Papadia P., Fanizzi F. Journal of Lipid Research, 2002, 43, 132 140. 2002_J_Lipid_res_Corcelli_et_al.pdf Articolo in rivista American Society for Biochemistry and Molecular Biology 0022 2275 Journal of lipid research Print Journal of lipid research Print J. lipid res. Print Journal of lipid research. Print JLR Print veronicamariateresa.lattanzio LATTANZIO VERONICA MARIA TERESA giuseppe.mascolo MASCOLO GIUSEPPE |