| Title | Microbial community dynamics during assays of harbour oil spill bioremediation: a microscale simulation study |
| Abstract | Aims: Microcosm experiments simulating an oil spill event were performed to evaluate the response of the natural microbial community structure of Messina harbour seawater following the accidental load of petroleum.
Methods and Results: An experimental harbour seawater microcosm, supplemented with nutrients and crude oil, was monitored above 15 days in comparison with unpolluted ones (control microcosms). Bacterial cells were counted with a Live/Dead BacLight viability kit; leucine aminopeptidase, beta-glucosidase, alkaline phosphatase, lipase and esterase enzymes were measured using fluorogenic substrates. The microbial community dynamic was monitored by isolation of total RNA, RT-PCR amplification of 16S rRNA, cloning and sequencing. Oil addition stimulated an increase of the total bacterial abundance, leucine aminopeptidase and phosphatase activity rates, as well as a change in the community structure. This suggested a prompt response of micro-organisms to the load of petroleum hydrocarbons.
Conclusions: The present study on the viability, specific composition and metabolic characteristics of the microbial community allows a more precise assessment of oil pollution. Both structural and functional parameters offer interesting perspectives as indicators to monitor changes caused by petroleum hydrocarbons.
Significance and Impact of the Study: A better knowledge of microbial structural successions at oil-polluted sites is essential for environmental bioremediation. Data obtained in microcosm studies improve our understanding of natural processes occurring during oil spills. |
| Source | Journal of applied microbiology (Print) 102, pp. 184–194 |
| Keywords | bioremediation; ectoenzymatic microbial activityharbourhydrocarbon-degrading bacteriamicrobial diversitymicrocosms |
| Journal | Journal of applied microbiology (Print) |
| Editor | Blackwell Science., Oxford, Regno Unito |
| Year | 2007 |
| Type | Articolo in rivista |
| DOI | 10.1111/j.1365-2672.2006.03071.x |
| Authors | Cappello S., Caruso G., Zampino D., Monticelli L.S, Maimone G., Denaro R., Tripodo B., Troussellier M. Yakimov M., and Giuliano L. |
| Text | 168424 2007 10.1111/j.1365 2672.2006.03071.x ISI Web of Science WOS WOS 000242832900021 Scopus 2 s2.0 33845641800 bioremediation; ectoenzymatic microbial activity harbour hydrocarbon degrading bacteria microbial diversity microcosms Microbial community dynamics during assays of harbour oil spill bioremediation a microscale simulation study Cappello S., Caruso G., Zampino D., Monticelli L.S, Maimone G., Denaro R., Tripodo B., Troussellier M. Yakimov M., and Giuliano L. CNR IAMC Messina Laboratoire Ecosystemes Lagunaires UMR_CNRS, Universite de Montpellier, France CNR ICTP Catania Aims Microcosm experiments simulating an oil spill event were performed to evaluate the response of the natural microbial community structure of Messina harbour seawater following the accidental load of petroleum. Methods and Results An experimental harbour seawater microcosm, supplemented with nutrients and crude oil, was monitored above 15 days in comparison with unpolluted ones control microcosms . Bacterial cells were counted with a Live/Dead BacLight viability kit; leucine aminopeptidase, beta glucosidase, alkaline phosphatase, lipase and esterase enzymes were measured using fluorogenic substrates. The microbial community dynamic was monitored by isolation of total RNA, RT PCR amplification of 16S rRNA, cloning and sequencing. Oil addition stimulated an increase of the total bacterial abundance, leucine aminopeptidase and phosphatase activity rates, as well as a change in the community structure. This suggested a prompt response of micro organisms to the load of petroleum hydrocarbons. Conclusions The present study on the viability, specific composition and metabolic characteristics of the microbial community allows a more precise assessment of oil pollution. Both structural and functional parameters offer interesting perspectives as indicators to monitor changes caused by petroleum hydrocarbons. Significance and Impact of the Study A better knowledge of microbial structural successions at oil polluted sites is essential for environmental bioremediation. Data obtained in microcosm studies improve our understanding of natural processes occurring during oil spills. 102 32 citazioni SCOPUS, 28 citazioni ISI, 40 citazioni Google Scholar, SJR 2011 0.864 SJR 2009 0.812, IF rivista 2009 2.098 oMicrobial community dynamics during assays of harbour oil spill bioremediation JAM2007.pdf Articolo in rivista Blackwell Science. 1364 5072 Journal of applied microbiology Print Journal of applied microbiology Print J. appl. microbiol. Print simone.cappello CAPPELLO SIMONE luissalvador.monticelli MONTICELLI LUIS SALVADOR gabriella.caruso CARUSO GABRIELLA danielaclotilde.zampino ZAMPINO DANIELA CLOTILDE laura.giuliano GIULIANO LAURA mikhail.iakimov IAKIMOV MIKHAIL renata.denaro DENARO RENATA giovanna.maimone MAIMONE GIOVANNA TA.P03.003.001 Qualita del Mare e delle Acque Costiere |
