Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Isolation and characterization of lipids strictly associated to PSII complexes: Focus on cardiolipin structural and functional role |
| Abstract | In this work, lipid extracts from spinach membrane fragments enriched in Photosystem II (PSII) and from spinach PSII dimers were analyzed, by means of Thin Layer Chromatography (TLC) and Electro-Spray Ionization Mass Spectrometry. Cardiolipin found in association with PSII was isolated and purified by preparative TLC, then characterized by mass and mass-mass analyses. Cardiolipin structures with four unsaturated C18 acyl chains and variable saturation degrees were evidenced. Structural and functional effects of different phospholipids on PSII complexes were investigated by Fluorescence, Resonance Light Scattering and Oxygen Evolution Rate measurements. An increment of PSII thermal stability was observed in the presence of cardiolipin and phosphatidylglycerol. |
| Source | Biochimica et biophysica acta. Biomembranes 1768, pp. 1620–1627 |
| Journal | Biochimica et biophysica acta. Biomembranes |
| Editor | Elsevier, Amsterdam, Paesi Bassi |
| Year | 2007 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.bbamem.2007.03.024 |
| Authors | Ventrella, L. Catucci, G. Mascolo, A. Corcelli, A. Agostiano |
| Text | 168163 2007 10.1016/j.bbamem.2007.03.024 Isolation and characterization of lipids strictly associated to PSII complexes Focus on cardiolipin structural and functional role Ventrella, L. Catucci, G. Mascolo, A. Corcelli, A. Agostiano a Dip. di Chimica, Universita di Bari, Via Orabona 4, 70126 Bari, Italy b IPCF CNR, sez. Bari, Via Orabona 4, 70126 Bari, Italy c IRSA CNR, sez. Bari, Via F. De Blasio 5, 70123 Bari, Italy d Dip. di Biochim. Medica e Biol. Medica e Fisica Medica, Univ. di Bari, P.zza G. Cesare, 70124 Bari, Italy In this work, lipid extracts from spinach membrane fragments enriched in Photosystem II PSII and from spinach PSII dimers were analyzed, by means of Thin Layer Chromatography TLC and Electro Spray Ionization Mass Spectrometry. Cardiolipin found in association with PSII was isolated and purified by preparative TLC, then characterized by mass and mass mass analyses. Cardiolipin structures with four unsaturated C18 acyl chains and variable saturation degrees were evidenced. Structural and functional effects of different phospholipids on PSII complexes were investigated by Fluorescence, Resonance Light Scattering and Oxygen Evolution Rate measurements. An increment of PSII thermal stability was observed in the presence of cardiolipin and phosphatidylglycerol. 1768 Isolation and characterization of lipids strictly associated to PSII complexes Focus on cardiolipin structural and functional role 2007_BBA_Catucci_.pdf Articolo in rivista Elsevier 0005 2736 Biochimica et biophysica acta. Biomembranes Biochimica et biophysica acta. Biomembranes Biochim. biophys. acta, Biomembr. Biochimica et biophysica acta. BBA. Biomembranes giuseppe.mascolo MASCOLO GIUSEPPE MD.P01.011.001 Strutture ad alta organizzazione gerarchica realizzate mediante approcci di tipo biologico e chimico, per lo studio e la progettazione di materiali e sistemi ibridi di interesse fotochimico TA.P07.002.002 Sviluppo di nuovi processi di controllo dell inquinamento idrico. |