| Title | Isolation and characterization of polyphenols-degrading bacteria from olive-mill wastewaters polluted soil |
| Abstract | In this study 28 bacterial strains, isolated from
greenwaters-polluted-soil, were investigated for their ability
to grow in presence of phenols added to Mineral Basal
Medium (MBM) in aerobic conditions. In particular, three
of them were found to be able to use as sole carbon source
phenol, cathecol, caffeic acid and ferulic acid with efficiency
ranging from 76% (phenol in 5 days, millimolar
concentration from 3.7 10-2 to 9 10-3) to 95% (ferulic acid
in 2 days millimolar concentration from 6.8 10-1 to 3
10-2). For these strains the taxonomic position was studied
by amplification and sequencing of 16S rRNA genes. The
isolated strains were classified belonging to Arthrobacter
sulfureus, Pseudomonas synxantha and Pseudomonas
oryzihabitans. Noteworthy, for the first time such Pseudomonas
strains have been shown to be able to use polyphenols
as the only carbon source in vitro. In fact, to the
best of our knowledge, this kind of study were not done on
Ps. Synxantha, while it was recently shown the ability of
P. oryzihabitans to degrade catechol. These findings may
open to new biotechnological applications for the degradation
of polyphenols. |
| Source | World journal of microbiology & biotechnology 26, pp. 639–647 |
| Journal | World journal of microbiology & biotechnology |
| Editor | Rapid Communications of Oxford Ltd. in association with UNESCO,, Oxford, Regno Unito |
| Year | 2010 |
| Type | Articolo in rivista |
| DOI | 10.1007/s11274-009-0217-x |
| Authors | Aresta M., Acquaviva M.I., Baruzzi F., Lo Noce R.M., Matarante A., Narracci M., Stabili L., Cavallo R. A. |
| Text | 166992 2010 10.1007/s11274 009 0217 x ISI Web of Science WOS 000275464900008 Isolation and characterization of polyphenols degrading bacteria from olive mill wastewaters polluted soil Aresta M., Acquaviva M.I., Baruzzi F., Lo Noce R.M., Matarante A., Narracci M., Stabili L., Cavallo R. A. Consorzio Interuniversitario Reattivita` Chimica e Catalisi, CIRCC, Via Celso Ulpiani, 27, 70126 Bari, Italy; CNR, IAMC, via Roma 3, 74100 Taranto, Italy; CNR, Institute of Sciences of Food Production, Via G. Amendola 122/O, 70125 Bari, Italy In this study 28 bacterial strains, isolated from greenwaters polluted soil, were investigated for their ability to grow in presence of phenols added to Mineral Basal Medium MBM in aerobic conditions. In particular, three of them were found to be able to use as sole carbon source phenol, cathecol, caffeic acid and ferulic acid with efficiency ranging from 76% phenol in 5 days, millimolar concentration from 3.7 10 2 to 9 10 3 to 95% ferulic acid in 2 days millimolar concentration from 6.8 10 1 to 3 10 2 . For these strains the taxonomic position was studied by amplification and sequencing of 16S rRNA genes. The isolated strains were classified belonging to Arthrobacter sulfureus, Pseudomonas synxantha and Pseudomonas oryzihabitans. Noteworthy, for the first time such Pseudomonas strains have been shown to be able to use polyphenols as the only carbon source in vitro. In fact, to the best of our knowledge, this kind of study were not done on Ps. Synxantha, while it was recently shown the ability of P. oryzihabitans to degrade catechol. These findings may open to new biotechnological applications for the degradation of polyphenols. 26 Articolo pubblicato 28 bacterial strains from greenwaters polluted soil were investigated for their ability to grow in presence of phenols. Three of them were found to be able to use as sole carbon source phenol, cathecol, caffeic acid and ferulic acid with efficiency ranging from 76% to 95%. The isolated strains, thanks to the analyses of partial 16S rRNA gene, were classified belonging to Arthrobacter sulfureus, Pseudomonas synxantha and Pseudomonas oryzihabitans. Noteworthy, for the first time such Pseudomonas strains have been shown to be able to use polyphenols as the only carbon source in vitro. These findings may open to new biotechnological applications for the degradation of polyphenols. Isolation_and_characterization_of_polyphenols_degrading_bacteria.pdf Articolo in rivista Rapid Communications of Oxford Ltd. in association with UNESCO, 0959 3993 World journal of microbiology biotechnology World journal of microbiology biotechnology World j. microbiol. biotechnol. World journal of microbiology biotechnology. World journal of microbiology and biotechnology WJMB loredana.stabili STABILI LOREDANA federico.baruzzi BARUZZI FEDERICO mariaimmacolata.acquaviva ACQUAVIVA MARIA IMMACOLATA marcella.narracci NARRACCI MARCELLA rosaanna.cavallo CAVALLO ROSA ANNA TA.P04.002.001 Fascia Costiera e Ambienti di Transizione analisi strutturale e funzionale delle componenti biotiche ed abiotiche degli ecosistemi per lo sviluppo sostenibile delle attivita AG.P05.016.001 Microbiologia e qualita delle produzioni alimentari |
