| Abstract | The reasons for the acidification problem affecting Food Waste (FW) anaerobic digestion were explored, combining the outcomes of microbiological data (FISH and CARD-FISH) and process modelling, based on the Anaerobic Digestion Model n°1 (ADM1). Long term semi continuous experiments were carried out, both with sole FW and with Waste Activated Sludge (WAS) as a co-substrate, at varying operational conditions (0.8-2.2 g VS L d) and FW / WAS ratios. Acidification was observed along FW mono-digestion, making it necessary to buffer the digesters; ADM1 modelling and experimental results suggested that this phenomenon was due to the methanogenic activity decline, most likely related to a deficiency in trace elements. WAS addition, even at proportions as low as 10% of the organic load, settled the acidification issue; this ability was related to the promotion of the methanogenic activity and the consequent enhancement of acetate consumption, rather than to WAS buffering capacity. The ability of the ADM1 to model processes affected by low microbial activity, such as FW mono-digestion, was also assessed. It was observed that the ADM1 was only adequate for digestions with a high activity level for both bacteria and methanogens (FISH/CARD-FISH ratio preferably >0.8) and, under these conditions, the model was able to correctly predict the relative abundance of both microbial populations, extrapolated from FISH analysis. |
| Text | 420308 2019 10.1016/j.wasman.2019.07.036 Scopus 2 s2.0 85069915561 Biogas ADM 1 Anaerobic co digestion Biowaste Acidification Microbial activity Anaerobic co digestion of food waste and waste activated sludge ADM1 modelling and microbial analysis to gain insights into the two substrates synergistic effects Montecchio D.; Astals S.; Di Castro V.; Gallipoli A.; Gianico A.; Pagliaccia P.; Piemonte V.; Rossetti S.; Tonanzi B.; Braguglia C.M. Istituto di Ricerca sulle Acque CNR, Area della Ricerca RM1, Monterotondo Roma , 00015, Istituto di Ricerca sulle Acque CNR, Area della Ricerca RM1, 00015 Monterotondo Roma , Italy, , Italy; Advanced Water Management Centre, The University of Queensland, Brisbane, QLD, 4072, Advanced Water Management Centre, The University of Queensland, Brisbane, QLD 4072, Australia, , Australia; Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, Barcelona, 08028, Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, 08028 Barcelona, Spain, , Spain; Department of Engineering, University Campus Bio medico of Rome, Roma, 00128, Department of Engineering, University Campus Bio medico of Rome, 00128 Roma, Italy, , Italy The reasons for the acidification problem affecting Food Waste FW anaerobic digestion were explored, combining the outcomes of microbiological data FISH and CARD FISH and process modelling, based on the Anaerobic Digestion Model n°1 ADM1 . Long term semi continuous experiments were carried out, both with sole FW and with Waste Activated Sludge WAS as a co substrate, at varying operational conditions 0.8 2.2 g VS L d and FW / WAS ratios. Acidification was observed along FW mono digestion, making it necessary to buffer the digesters; ADM1 modelling and experimental results suggested that this phenomenon was due to the methanogenic activity decline, most likely related to a deficiency in trace elements. WAS addition, even at proportions as low as 10% of the organic load, settled the acidification issue; this ability was related to the promotion of the methanogenic activity and the consequent enhancement of acetate consumption, rather than to WAS buffering capacity. The ability of the ADM1 to model processes affected by low microbial activity, such as FW mono digestion, was also assessed. It was observed that the ADM1 was only adequate for digestions with a high activity level for both bacteria and methanogens FISH/CARD FISH ratio preferably >0.8 and, under these conditions, the model was able to correctly predict the relative abundance of both microbial populations, extrapolated from FISH analysis. 97 Published version http //www.scopus.com/record/display.url eid=2 s2.0 85069915561 origin=inward Anaerobic co digestion of food waste and waste activated sludge ADM1 modelling and microbial analysis to gain insights paper 3 Anaerobic co digestion of FW and WAS 2019.pdf Articolo in rivista Pergamon Press, 0956 053X Waste management Elmsford Waste management Elmsford Waste management. Elmsford PAGLIACCIA PAMELA TONANZI BARBARA simona.rossetti ROSSETTI SIMONA camillamaria.braguglia BRAGUGLIA CAMILLA MARIA andrea.gianico GIANICO ANDREA daniele.montecchio MONTECCHIO DANIELE agata.gallipoli GALLIPOLI AGATA |
