Scheda di dettaglio – i prodotti della ricerca
| Dato | Valore |
|---|---|
| Title | Using Real-time PCR to assess changes in the crude oil degrading microbial community in contaminated seawater mesocosms |
| Abstract | The real-time polymerase chain reaction (RT-PCR) was used to follow changes in the proportion of hydrocarbonoclastic bacteria in the marine microbial community in oil polluted mesocosms during bioremediation field trial. Assay for alk-B1 of Alcanivorax borkumensis and alk-BT of Thalassolituus oleivorans were validated and found to be both sensitive and reproducible. Quantification of alk-B1 from A.borkumensis SK2 in mesocosms show that in single bioaugmentation mesocosm (M1) this gene has high quantity in fifth day of sampling but in biostimulating mesocosm (M2) and consortium bioaugmentation mesocosm (M3) the high quantity of this gene was in tenth day of sampling. The comparison between expression of alk-BT and alk-B1 in M3 mesocosm show that alk-B1 copy number was more than alk-BT. The proportion of alk-B1 or alk-BT containing bacteria was positively correlated to the concentration of crude oil in the mesocosms. After the concentration of crude oil in the mesocosms decreased the gene copy number of alkane monooxygenase genes also decreased. © 2014 Elsevier Ltd. |
| Source | International biodeterioration & biodegradation 93, pp. 241–248 |
| Keywords | BiodegradationCrude-oilExpressionMesocosmQuantification |
| Journal | International biodeterioration & biodegradation |
| Editor | Elsevier Applied Science,, Barking, Regno Unito |
| Year | 2014 |
| Type | Articolo in rivista |
| DOI | 10.1016/j.ibiod.2014.06.006 |
| Authors | Hassanshahian, Mehdi; Yakimov, Michail M.; Denaro, Renata; Genovese, Maria; Cappello, Simone |
| Text | 312608 2014 10.1016/j.ibiod.2014.06.006 Scopus 2 s2.0 84903847555 Biodegradation Crude oil Expression Mesocosm Quantification Using Real time PCR to assess changes in the crude oil degrading microbial community in contaminated seawater mesocosms Hassanshahian, Mehdi; Yakimov, Michail M.; Denaro, Renata; Genovese, Maria; Cappello, Simone Daneshgahe Shahid Bahonar e Kerman; Istituto per l Ambiente Marino Costiero del Consiglio Nazionale delle Ricerche IAMC CNR The real time polymerase chain reaction RT PCR was used to follow changes in the proportion of hydrocarbonoclastic bacteria in the marine microbial community in oil polluted mesocosms during bioremediation field trial. Assay for alk B1 of Alcanivorax borkumensis and alk BT of Thalassolituus oleivorans were validated and found to be both sensitive and reproducible. Quantification of alk B1 from A.borkumensis SK2 in mesocosms show that in single bioaugmentation mesocosm M1 this gene has high quantity in fifth day of sampling but in biostimulating mesocosm M2 and consortium bioaugmentation mesocosm M3 the high quantity of this gene was in tenth day of sampling. The comparison between expression of alk BT and alk B1 in M3 mesocosm show that alk B1 copy number was more than alk BT. The proportion of alk B1 or alk BT containing bacteria was positively correlated to the concentration of crude oil in the mesocosms. After the concentration of crude oil in the mesocosms decreased the gene copy number of alkane monooxygenase genes also decreased. © 2014 Elsevier Ltd. 93 http //www.scopus.com/record/display.url eid=2 s2.0 84903847555 origin=inward Articolo in rivista Elsevier Applied Science, 0964 8305 International biodeterioration biodegradation International biodeterioration biodegradation Int. biodeterior. biodegrad. International biodeterioration and biodegradation. mikhail.iakimov IAKIMOV MIKHAIL renata.denaro DENARO RENATA maria.genovese GENOVESE MARIA |